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T7p07 RNA polymerase [ Escherichia phage T7 ]

Gene ID: 1261050, updated on 18-Aug-2023

Summary

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Gene symbol
T7p07
Gene description
RNA polymerase
Locus tag
T7p07
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia phage T7 (nat-host: Escherichia coli)
Lineage
Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Autographiviridae; Studiervirinae; Teseptimavirus
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Genomic context

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Sequence:
NC_001604.1 (3139..5887)

NC_001604.1Genomic Context describing neighboring genes Neighboring gene ocr-like anti-restriction Neighboring gene hypothetical protein Neighboring gene serine-threonine kinase Neighboring gene hypothetical protein Neighboring gene hypothetical protein Neighboring gene hypothetical protein Neighboring gene dGTPase inhibitor; target for F exclusion Neighboring gene hypothetical protein Neighboring gene DNA ligase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001604.1

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Investigation of specific interactions between T7 promoter and T7 RNA polymerase by force spectroscopy using atomic force microscope. Zhang X, et al. Biochem J, 2018 Jan 11. PMID 29187520
  2. Nucleotide sequence of the gene for bacteriophage T7 RNA polymerase. Moffatt BA, et al. J Mol Biol, 1984 Feb 25. PMID 6708104
  3. T7 RNA polymerase-directed expression of an antibody fragment transgene in plastids causes a semi-lethal pale-green seedling phenotype. Magee AM, et al. Transgenic Res, 2004 Aug. PMID 15517992
  4. Weakening of the T7 promoter-polymerase interaction facilitates promoter release. Guo Q, et al. J Biol Chem, 2005 Apr 15. PMID 15711016
  5. A mutation in T7 RNA polymerase that facilitates promoter clearance. Guillerez J, et al. Proc Natl Acad Sci U S A, 2005 Apr 26. PMID 15831591, Free PMC Article

See all (32) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data indicate that the RNA polymerase of the bacteriophage T7 has a number of appealing properties for synthetic biological designs.
    Title: Programmable T7-based synthetic transcription factors.
  2. Using correlation analysis, it was found that the promoter strength characterized by reporter gene expression was closely correlated with rupture force and the binding percentage of T7 RNA polymerase by force spectroscopy.
    Title: Investigation of specific interactions between T7 promoter and T7 RNA polymerase by force spectroscopy using atomic force microscope.
  3. From the docking of the minimum energy representative structures of T7 lysozyme at different pH strengths (obtained from the free energy landscape analysis) with T7RNAP structures at same pH strengths, we saw strong interaction patterns at pH 7.9 and pH 5.
    Title: A computational assessment of pH-dependent differential interaction of T7 lysozyme with T7 RNA polymerase.
  4. Data suggest that comparative transcriptional fidelities/mutation rates for DNA-directed RNA polymerases are as follows: human mitochondrial POLRMT [2x10(-5)]; Saccharomyces cerevisiae mitochondrial Rpo41 [6x10(-6)]; phage T7 single-subunit RNA polymerase [2x10(-6)].
    Title: Transcriptional fidelities of human mitochondrial POLRMT, yeast mitochondrial Rpo41, and phage T7 single-subunit RNA polymerases.
  5. Molecular dynamics simulation study described the behavior of the two magnesium ions in RNAP activity during genetic transcription.
    Title: Molecular dynamics simulation study of the "stay or leave" problem for two magnesium ions in gene transcription.
  6. The control of the discrimination between dNTP and rNTP in DNA and RNA polymerases has been described.
    Title: The control of the discrimination between dNTP and rNTP in DNA and RNA polymerase.
  7. The study provides evidence that the intrinsic infidelity of T7 RNAP in transcription brings significant phenotypic consequences, observed as frameshift mutation restoration and the production of a heterogenous population of the full-length protein.
    Title: RNA editing by T7 RNA polymerase bypasses InDel mutations causing unexpected phenotypic changes.
  8. We show here that DNA-protein cross-links constitute strong but not absolute blocks to in vitro transcription catalyzed by T7 RNAP.
    Title: T7 RNA polymerases backed up by covalently trapped proteins catalyze highly error prone transcription.
  9. Hydrogen bonds of RNA polymerase play an important role in the efficiency of transcription.
    Title: Importance of steric effects on the efficiency and fidelity of transcription by T7 RNA polymerase.
  10. study reports crystal structures of T7 RNAP bound to promoter DNA containing either a 7- or an 8-nucleotide (nt) RNA transcript that illuminate intermediate states along the transition pathway
    Title: The structure of a transcribing T7 RNA polymerase in transition from initiation to elongation.
Submit: New GeneRIF Correction See all GeneRIFs (20)

General gene information

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Homology

misc_structure

  • Loc: 5887

regulatory

  • Loc: 5848 regulatory_class = promoter

General protein information

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Preferred Names
RNA polymerase
NP_041960.1
  • A family of single subunit RNA polymerases.

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001604.1 Reference assembly

    Range
    3139..5887
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_041960.1 RNA polymerase [Escherichia phage T7]

    See identical proteins and their annotated locations for NP_041960.1

    Status: PROVISIONAL

    UniProtKB/Swiss-Prot
    P00573, Q38543
    UniProtKB/TrEMBL
    A0A8F3HLW0, C1KTT1, Q3V7E5, Q6WYR7
    Conserved Domains (1) summary
    PHA00452
    Location:28 → 883
    PHA00452; T3/T7-like RNA polymerase
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
1CEZ_A.1
1H38_A.1
1H38_B.1
1H38_C.1
1H38_D.1
1MSW_D.1
1QLN_A.1
1S0V_A.1
1S0V_B.1
1S0V_C.1
1S0V_D.1
1S76_D.1
1S77_D.1
P00573.2

Gene LinkOut

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Molecular Biology Databases
Research Materials