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    amtB ammonium transporter [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945084, updated on 10-Oct-2024

    Summary

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    Official Symbol
    amtB
    Official Full Name
    ammonium transporter
    Primary source
    ECOCYC:EG11821
    Locus tag
    b0451
    See related
    ASAP:ABE-0001564
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0445; ybaG
    Summary
    AmtB is a trimeric inner membrane protein. [More information is available at EcoGene: EG11821]. AmtB, a member of the Amt family of ammonium/ammonia transporters, is responsible for the bidirectional diffusion of ammonium across the membrane and is necessary for growth only at low ammonium concentrations. [More information is available at EcoCyc: EG11821].
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    Genomic context

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    Sequence:
    NC_000913.3 (472966..474252)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene ABC transporter family protein MdlA Neighboring gene ABC transporter family protein MdlB Neighboring gene nitrogen regulatory protein PII-2 Neighboring gene acyl-CoA thioesterase II Neighboring gene PF09619 family lipoprotein YbaY

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Molecular determinants for binding of ammonium ion in the ammonia transporter AmtB-A quantum chemical analysis. Liu Y, et al. J Phys Chem A, 2006 Feb 2. PMID 16435797
    2. The Amt/MEP/Rh family: structure of AmtB and the mechanism of ammonia gas conduction. Khademi S, et al. Physiology (Bethesda), 2006 Dec. PMID 17119155
    3. Detailed mechanism for AmtB conducting NH4+/NH3: molecular dynamics simulations. Yang H, et al. Biophys J, 2007 Feb 1. PMID 17098799, Free PMC Article
    4. Ammonium recruitment and ammonia transport by E. coli ammonia channel AmtB. Nygaard TP, et al. Biophys J, 2006 Dec 15. PMID 17012311, Free PMC Article
    5. Computational study of the binding affinity and selectivity of the bacterial ammonium transporter AmtB. Luzhkov VB, et al. Biochemistry, 2006 Sep 12. PMID 16953566

    See all (67) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. simulations reveal previously undiscovered POPG binding sites on the intracellular side of the lipid bilayer between the AmtB subunits.
      Title: The lipid environment determines the activity of the Escherichia coli ammonium transporter AmtB.
    2. Amt and Rh proteins are not functionally equivalent and that permeation takes place according to two distinct mechanisms.
      Title: Different hydration patterns in the pores of AmtB and RhCG could determine their transport mechanisms.
    3. the twin-histidine element serves as a filter to prevent K(+) conduction in Escherichia coli ammonium channel AmtB
      Title: The molecular basis of K+ exclusion by the Escherichia coli ammonium channel AmtB.
    4. Data explore the similarity and difference of the conduction mechanism of methylamine and ammonia molecules through AmtB, using molecular dynamics simulations.
      Title: Molecular dynamics simulations on the mechanism of transporting methylamine and ammonia by ammonium transporter AmtB.
    5. AmtB activity absolutely requires F215 but not F107
      Title: Substrate binding, deprotonation, and selectivity at the periplasmic entrance of the Escherichia coli ammonia channel AmtB.
    6. AmtB allows accumulation of CH(3)NH(3)(+) ion in response to the electrical potential across the membrane
      Title: The W148L substitution in the Escherichia coli ammonium channel AmtB increases flux and indicates that the substrate is an ion.
    7. N-terminus verified by Edman degradation on mature peptide
      Title: The ammonia channel protein AmtB from Escherichia coli is a polytopic membrane protein with a cleavable signal peptide.
    8. The GlnK protein binding causes a significant downshift in the Am6 pK(a) value of the AmtB. However, this downshift is perfectly compensated by the reorientation of the protein backbone upon AmtB-GlnK complex formation.
      Title: Modulation of the protein environment in the hydrophilic pore of the ammonia transporter protein AmtB upon GlnK protein binding.
    9. Co-purification of AmtB with GlnK from ammonium-shocked E. coli cells definitvely demonstrates that these two proteins form a functional complex with a stoichiometry of 1:1.
      Title: In vitro analysis of the Escherichia coli AmtB-GlnK complex reveals a stoichiometric interaction and sensitivity to ATP and 2-oxoglutarate.
    10. simulations showed that the entrance of NH(4)(+) into the periplasmic recruitment vestibule requires only 3.1 kcal/mol of energy
      Title: Molecular dynamics simulations on the Escherichia coli ammonia channel protein AmtB: mechanism of ammonia/ammonium transport.
    Submit: New GeneRIF Correction See all GeneRIFs (16)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ammonium channel activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ammonium channel activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ammonium channel activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in ammonium transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ammonium transmembrane transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in ammonium transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in carbon dioxide transport IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    ammonium transporter

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      472966..474252
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414985.1 ammonium transporter [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414985.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0AAQ2LTE2
      Conserved Domains (1) summary
      PRK10666
      Location:1 → 428
      PRK10666; ammonium transporter AmtB
    Nucleotide Protein
    Heading Accession and Version

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