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    SRB4 Srb4p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 856743, updated on 2-Nov-2024

    Summary

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    Official Symbol
    SRB4
    Official Full Name
    Srb4p
    Primary source
    SGD:S000000824
    Locus tag
    YER022W
    See related
    AllianceGenome:SGD:S000000824; FungiDB:YER022W; VEuPathDB:YER022W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    MED17
    Summary
    Enables DNA-binding transcription factor binding activity; RNA polymerase II complex recruiting activity; and RNA polymerase II core promoter sequence-specific DNA binding activity. Involved in RNA polymerase II preinitiation complex assembly; cellular response to heat; and positive regulation of transcription by RNA polymerase II. Part of core mediator complex. Used to study infantile cerebral and cerebellar atrophy with postnatal progressive microcephaly. Human ortholog(s) of this gene implicated in infantile cerebral and cerebellar atrophy with postnatal progressive microcephaly. Orthologous to human MED17 (mediator complex subunit 17). [provided by Alliance of Genome Resources, Nov 2024]
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    Genomic context

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    See SRB4 in Genome Data Viewer
    Location:
    chromosome: V
    Exon count:
    1
    Sequence:
    Chromosome: V; NC_001137.3 (198812..200875)

    Chromosome V - NC_001137.3Genomic Context describing neighboring genes Neighboring gene guanine nucleotide-binding protein subunit alpha Neighboring gene proteasome regulatory particle lid subunit RPN3 Neighboring gene pyrroline-5-carboxylate reductase Neighboring gene carnitine O-acetyltransferase YAT2

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001137.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Mediator complex association with constitutively transcribed genes in yeast. Ansari SA, et al. Proc Natl Acad Sci U S A, 2009 Sep 29. PMID 19805365, Free PMC Article
    2. Direct interaction of RNA polymerase II and mediator required for transcription in vivo. Soutourina J, et al. Science, 2011 Mar 18. PMID 21415355
    3. Transcriptional activation independent of TFIIH kinase and the RNA polymerase II mediator in vivo. Lee D, et al. Nature, 1998 May 28. PMID 9620805
    4. Infantile cerebral and cerebellar atrophy is associated with a mutation in the MED17 subunit of the transcription preinitiation mediator complex. Kaufmann R, et al. Am J Hum Genet, 2010 Nov 12. PMID 20950787, Free PMC Article
    5. Contact with a component of the polymerase II holoenzyme suffices for gene activation. Barberis A, et al. Cell, 1995 May 5. PMID 7736588

    See all (126) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Architectural Mediator subunits are differentially essential for global transcription in Saccharomyces cerevisiae.
      Title: Architectural Mediator subunits are differentially essential for global transcription in Saccharomyces cerevisiae.
    2. Data show that Med17(Srb4) are direct targets of mediator histone H4 tail interactions.
      Title: Med5(Nut1) and Med17(Srb4) are direct targets of mediator histone H4 tail interactions.
    3. identifed direct physical interaction between the Rpb3 Pol II subunit and the Mediator subunit, Med17; findings suggest a direct interaction between Mediator and Pol II is generally required for transcription of class II genes in eukaryotes
      Title: Direct interaction of RNA polymerase II and mediator required for transcription in vivo.
    4. identified as a general transcription factor, comparable in importance to RNA polymerase II and other general factors for the initiation of transcription
      Title: Mediator as a general transcription factor.
    5. characterize Mediator from cells carrying the srb4-138 allele and find that the Mediator complex consistently breaks apart at the head/middle domain boundary even at lower temperatures
      Title: The classical srb4-138 mutant allele causes dissociation of yeast Mediator.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA-binding transcription factor binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA polymerase II complex recruiting activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables RNA polymerase II complex recruiting activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables RNA polymerase II core promoter sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables transcription coregulator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transcription coregulator activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in RNA polymerase II preinitiation complex assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cellular response to heat IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of transcription by RNA polymerase II IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transcription initiation at RNA polymerase II promoter IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of core mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of core mediator complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of mediator complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    Srb4p
    NP_010939.1
    • Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for basal RNA polymerase II transcription; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001137.3 Reference assembly

      Range
      198812..200875
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178913.1 → NP_010939.1  TPA: Srb4p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010939.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D3DLS1, P32569, Q06790, Q6B1B4
      UniProtKB/TrEMBL
      A6ZQX1, G2WCQ5
      Conserved Domains (1) summary
      pfam10156
      Location:86 → 569
      Med17; Subunit 17 of Mediator complex
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P32569.1

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