SMC3 cohesin subunit SMC3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SMC3
Official Full Name: cohesin subunit SMC3
Primary source: SGD:S000003610
Locus tag: YJL074C
See related: AllianceGenome:SGD:S000003610; FungiDB:YJL074C; VEuPathDB:YJL074C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables protein kinase binding activity. Contributes to ATP hydrolysis activity. Involved in DNA recombination; G2/M transition of mitotic cell cycle; and chromosome organization. Located in nucleus. Part of mitotic cohesin complex. Human ortholog(s) of this gene implicated in Cornelia de Lange syndrome 3; hepatocellular carcinoma; and intellectual disability. Orthologous to human SMC3 (structural maintenance of chromosomes 3). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See SMC3 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (299157..302849, complement)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Conformational dynamics of cohesin/Scc2 loading complex are regulated by Smc3 acetylation and ATP binding.
Title: Conformational dynamics of cohesin/Scc2 loading complex are regulated by Smc3 acetylation and ATP binding.
A model for Scc2p stimulation of cohesin's ATPase and its inhibition by acetylation of Smc3p.
Title: A model for Scc2p stimulation of cohesin's ATPase and its inhibition by acetylation of Smc3p.
DNA passes through cohesin's hinge as well as its Smc3-kleisin interface.
Title: DNA passes through cohesin's hinge as well as its Smc3-kleisin interface.
Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin-dependent chromatin loops.
Title: Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin-dependent chromatin loops.
The Interplay of Cohesin and the Replisome at Processive and Stressed DNA Replication Forks.
Title: The Interplay of Cohesin and the Replisome at Processive and Stressed DNA Replication Forks.
Functional control of Eco1 through the MCM complex in sister chromatid cohesion.
Title: Functional control of Eco1 through the MCM complex in sister chromatid cohesion.
ATP-binding and Smc1-Smc3 heterodimerization promote conformational changes within the ATPase that are transmitted to the Smc coiled-coil domains.
Title: The structure of the cohesin ATPase elucidates the mechanism of SMC-kleisin ring opening.
The authors show here that Scc2 also actively maintains cohesin on chromosomes during G1 in S. cerevisiae cells. It does so by blocking a Wapl-independent release reaction that requires opening the cohesin ring at its Smc3/Scc1 interface as well as the D loop of Smc1's ATPase.
Title: Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1.
The X. laevis Eco2 bound to its primary and secondary Smc3 substrates demonstrate the plasticity of the substrate-binding site, which confers substrate specificity by concerted conformational changes of the central beta-hairpin and the C-terminal extension.
Title: Structural Basis of Eco1-Mediated Cohesin Acetylation.
Smc3 deacetylation by Hos1, triggered by Scc1 cleavage, facilitates efficient dissolution of sister chromatid cohesion during early metaphase.
Title: Smc3 Deacetylation by Hos1 Facilitates Efficient Dissolution of Sister Chromatid Cohesion during Early Anaphase.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
contributes_to ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in G2/M transition of mitotic cell cycle	IPI Inferred from Physical Interaction more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromosome organization	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in meiotic sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid cohesion	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic sister chromatid cohesion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in replication-born double-strand break repair via sister chromatid exchange	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptonemal complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
part_of mitotic cohesin complex	IBA Inferred from Biological aspect of Ancestor more info
part_of mitotic cohesin complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: cohesin subunit SMC3

NP_012461.1

Subunit of the multiprotein cohesin complex; required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

299157..302849 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181507.1 → NP_012461.1 TPA: cohesin subunit SMC3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012461.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWA9, P47037

UniProtKB/TrEMBL

B3LQ37, N1P196

Conserved Domains (1) summary

COG1196
Location:1 → 1221

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]