Ubash3b ubiquitin associated and SH3 domain containing, B [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ubash3bprovided by MGI
Official Full Name: ubiquitin associated and SH3 domain containing, Bprovided by MGI
Primary source: MGI:MGI:1920078
See related: Ensembl:ENSMUSG00000032020 AllianceGenome:MGI:1920078
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: p70; STS-1; TULA-2; 2810457I06Rik
Summary: Enables phosphoprotein binding activity and protein tyrosine phosphatase activity. Involved in regulation of osteoclast differentiation. Acts upstream of or within several processes, including collagen-activated tyrosine kinase receptor signaling pathway; negative regulation of bone resorption; and negative regulation of platelet aggregation. Predicted to be located in nucleus. Predicted to be active in cytoplasm. Is expressed in several structures, including central nervous system; incisor; limb; nasal cavity epithelium; and retina. Orthologous to human UBASH3B (ubiquitin associated and SH3 domain containing B). [provided by Alliance of Genome Resources, Oct 2024]
Expression: Broad expression in cerebellum adult (RPKM 9.2), CNS E18 (RPKM 4.0) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 A5.1; 9 21.84 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (40922056..41069358, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (41010760..41161692, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Bone marrow-derived dendritic cells (BMDCs) lacking Protein Tyrosine Phosphatase Sts-1 expression have an enhanced ability to inhibit Candida albicans growth and contribute significantly to the resistance of Sts-/- mice. Within BMDCs, the Sts phosphatases negatively regulate the activation of select pathways downstream of the key fungal pathogen pattern recognition receptor Dectin-1.
Title: Phagocytes from Mice Lacking the Sts Phosphatases Have an Enhanced Antifungal Response to Candida albicans.
Data suggest that Sts1 (suppressor of T-cell receptor signaling-1) negatively regulates TCR (T-cell receptor) signaling in activated T-lymphocytes via dephosphorylation of Zap70.
Title: Insights into the suppressor of T-cell receptor (TCR) signaling-1 (Sts-1)-mediated regulation of TCR signaling through the use of novel substrate-trapping Sts-1 phosphatase variants.
Sts-1 and Sts-2 are not required for the immune control of murine herpesvirus 68 in a normal course of gammaherpesvirus infection.
Title: Enhanced response of T cells from murine gammaherpesvirus 68-infected mice lacking the suppressor of T cell receptor signaling molecules Sts-1 and Sts-2.
The absence of TULA-2 and also the relative level of TULA-2 expression modulates FcgammaRIIA-mediated platelet reactivity and heparin-induced thrombocytopenia in vivo.
Title: TULA-2 (T-Cell Ubiquitin Ligand-2) Inhibits the Platelet Fc Receptor for IgG IIA (FcγRIIA) Signaling Pathway and Heparin-Induced Thrombocytopenia in Mice.
dephosphorylation of Tyr(P)(346) may be considered an important "checkpoint" in the regulation of Syk activation process. Putative biological functions of TULA-2-mediated dephosphorylation of Tyr(P)(346) may include deactivation of receptor-activated Syk or suppression of Syk activation by suboptimal stimulation.
Title: TULA-2 Protein Phosphatase Suppresses Activation of Syk through the GPVI Platelet Receptor for Collagen by Dephosphorylating Tyr(P)346, a Regulatory Site of Syk.
Biochemical analysis identified STS-1 and STS-2 as direct phosphatases of FLT3 and c-KIT.
Title: The Phosphatases STS1 and STS2 Regulate Hematopoietic Stem and Progenitor Cell Fitness.
results demonstrate the importance of STS-1 in regulating IFN-alpha-induced autophagy in B cells.
Title: STS-1 promotes IFN-α induced autophagy by activating the JAK1-STAT1 signaling pathway in B cells.
The lack of TULA-2 facilitates T-cell responses to TCR/CD3 stimulation, thus exacerbating T-cell-dependent inflammation.
Title: Members of the novel UBASH3/STS/TULA family of cellular regulators suppress T-cell-driven inflammatory responses in vivo.
TULA-2 negatively regulates osteoclast differentiation and function.
Title: TULA-2, a novel histidine phosphatase, regulates bone remodeling by modulating osteoclast function.
Once phosphorylated, tyrosines in carboxyl terminus of protein-tyrosine kinase Syk interact with signaling proteins, including TULA-2, a negative regulator of mast cell degranulation.
Title: Once phosphorylated, tyrosines in carboxyl terminus of protein-tyrosine kinase Syk interact with signaling proteins, including TULA-2, a negative regulator of mast cell degranulation.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (1) 1 citation
Transposon induced (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D9Mit286 (e-PCR), detects polymorphism
- UniSTS:131855

NoName (e-PCR)
- UniSTS:236100

UniSTS:236101 (e-PCR)
- UniSTS:236101

BB125008 (e-PCR)
- UniSTS:185784

AU049662 (e-PCR)
- UniSTS:146108

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables phosphoprotein binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein tyrosine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein tyrosine phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein tyrosine phosphatase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ubiquitin protein ligase binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within collagen-activated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in collagen-activated tyrosine kinase receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within collagen-activated tyrosine kinase receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of bone resorption	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within negative regulation of bone resorption	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of osteoclast differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of platelet aggregation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within negative regulation of signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within peptidyl-tyrosine dephosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within peptidyl-tyrosine dephosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within platelet activation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in platelet aggregation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within platelet aggregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of osteoclast differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of osteoclast differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of protein binding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of release of sequestered calcium ion into cytosol	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of release of sequestered calcium ion into cytosol	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ubiquitin-associated and SH3 domain-containing protein B

Names: T-cell ubiquitin ligand 2; cbl-interacting protein p70; suppressor of T-cell receptor signaling 1; tyrosine-protein phosphatase STS1/TULA2

NP_001344341.1

EC 3.1.3.48

NP_789830.1

EC 3.1.3.48

XP_006510695.1

EC 3.1.3.48

XP_006510699.1

EC 3.1.3.48

XP_017169101.1

EC 3.1.3.48

XP_030100498.1

EC 3.1.3.48

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001357412.1 → NP_001344341.1 ubiquitin-associated and SH3 domain-containing protein B isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.

Source sequence(s)

AC159820, AK013361

Consensus CDS

CCDS90541.1

Related

ENSMUSP00000116038.2, ENSMUST00000151485.8

Conserved Domains (2) summary

cd07067
Location:253 → 465

HP_PGM_like; Histidine phosphatase domain found in phosphoglycerate mutases and related proteins, mostly phosphatases; contains a His residue which is phosphorylated during the reaction

cd11936
Location:123 → 184

SH3_UBASH3B; Src homology 3 domain of Ubiquitin-associated and SH3 domain-containing protein B
NM_176860.6 → NP_789830.1 ubiquitin-associated and SH3 domain-containing protein B isoform 1

See identical proteins and their annotated locations for NP_789830.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC159820, AK035764, AK150909

Consensus CDS

CCDS23085.1

UniProtKB/Swiss-Prot

Q3U8Z2, Q8BGG7, Q8BMW9

Related

ENSMUSP00000043865.9, ENSMUST00000044155.15

Conserved Domains (3) summary

cd07067
Location:375 → 587

HP_PGM_like; Histidine phosphatase domain found in phosphoglycerate mutases and related proteins, mostly phosphatases; contains a His residue which is phosphorylated during the reaction

cd11936
Location:245 → 306

SH3_UBASH3B; Src homology 3 domain of Ubiquitin-associated and SH3 domain-containing protein B

cd14301
Location:28 → 65

UBA_UBS3B; UBA domain found in ubiquitin-associated and SH3 domain-containing protein B (UBS3B) and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

40922056..41069358 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006510632.2 → XP_006510695.1 ubiquitin-associated and SH3 domain-containing protein B isoform X1

Conserved Domains (3) summary

cd07067
Location:516 → 728

HP_PGM_like; Histidine phosphatase domain found in phosphoglycerate mutases and related proteins, mostly phosphatases; contains a His residue which is phosphorylated during the reaction

cd11936
Location:386 → 447

SH3_UBASH3B; Src homology 3 domain of Ubiquitin-associated and SH3 domain-containing protein B

PRK12323
Location:66 → 164

PRK12323; DNA polymerase III subunit gamma/tau
XM_006510636.5 → XP_006510699.1 ubiquitin-associated and SH3 domain-containing protein B isoform X2

Conserved Domains (4) summary

cd07067
Location:352 → 564

HP_PGM_like; Histidine phosphatase domain found in phosphoglycerate mutases and related proteins, mostly phosphatases; contains a His residue which is phosphorylated during the reaction

cd11936
Location:222 → 283

SH3_UBASH3B; Src homology 3 domain of Ubiquitin-associated and SH3 domain-containing protein B

COG5207
Location:6 → 52

UBP14; Uncharacterized Zn-finger protein, UBP-type [General function prediction only]

cl21463
Location:21 → 42

UBA_like_SF; UBA domain-like superfamily
XM_017313612.2 → XP_017169101.1 ubiquitin-associated and SH3 domain-containing protein B isoform X3

UniProtKB/Swiss-Prot

Q8BGG7

Conserved Domains (2) summary

cd07067
Location:253 → 465

HP_PGM_like; Histidine phosphatase domain found in phosphoglycerate mutases and related proteins, mostly phosphatases; contains a His residue which is phosphorylated during the reaction

cd11936
Location:123 → 184

SH3_UBASH3B; Src homology 3 domain of Ubiquitin-associated and SH3 domain-containing protein B
XM_030244638.1 → XP_030100498.1 ubiquitin-associated and SH3 domain-containing protein B isoform X3

Conserved Domains (2) summary

cd07067
Location:253 → 465

HP_PGM_like; Histidine phosphatase domain found in phosphoglycerate mutases and related proteins, mostly phosphatases; contains a His residue which is phosphorylated during the reaction

cd11936
Location:123 → 184

SH3_UBASH3B; Src homology 3 domain of Ubiquitin-associated and SH3 domain-containing protein B