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    Lima1 LIM domain and actin binding 1 [ Mus musculus (house mouse) ]

    Gene ID: 65970, updated on 28-Oct-2024

    Summary

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    Official Symbol
    Lima1provided by MGI
    Official Full Name
    LIM domain and actin binding 1provided by MGI
    Primary source
    MGI:MGI:1920992
    See related
    Ensembl:ENSMUSG00000023022 AllianceGenome:MGI:1920992
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Eplin
    Summary
    Enables actin filament binding activity. Involved in several processes, including cholesterol homeostasis; intestinal cholesterol absorption; and ruffle organization. Located in actin cytoskeleton; brush border membrane; and ruffle. Is expressed in several structures, including alimentary system; brain; genitourinary system; immune system; and respiratory system. Orthologous to human LIMA1 (LIM domain and actin binding 1). [provided by Alliance of Genome Resources, Oct 2024]
    Expression
    Broad expression in bladder adult (RPKM 44.8), colon adult (RPKM 23.3) and 27 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Lima1 in Genome Data Viewer
    Location:
    15 F1; 15 56.13 cM
    Exon count:
    13
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 15 NC_000081.7 (99676351..99773292, complement)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 15 NC_000081.6 (99778468..99875477, complement)

    Chromosome 15 - NC_000081.7Genomic Context describing neighboring genes Neighboring gene CapStarr-seq enhancer MGSCv37_chr15:99555871-99556054 Neighboring gene cytochrome c oxidase assembly protein 14 Neighboring gene ceramide synthase 5 Neighboring gene VISTA enhancer mm17 Neighboring gene predicted gene, 25897 Neighboring gene 60S ribosomal protein L37 pseudogene Neighboring gene predicted gene 17057 Neighboring gene STARR-seq mESC enhancer starr_39724 Neighboring gene STARR-seq mESC enhancer starr_39726 Neighboring gene STARR-seq mESC enhancer starr_39729 Neighboring gene predicted gene, 18095 Neighboring gene phosphoribosyl pyrophosphate synthetase 1 pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Lipotoxic hepatocyte derived LIMA1 enriched small extracellular vesicles promote hepatic stellate cells activation via inhibiting mitophagy. Li S, et al. Cell Mol Biol Lett, 2024 May 31. PMID 38822260, Free PMC Article
    2. Inhibition of anchorage-independent growth of transformed NIH3T3 cells by epithelial protein lost in neoplasm (EPLIN) requires localization of EPLIN to actin cytoskeleton. Song Y, et al. Mol Biol Cell, 2002 Apr. PMID 11950948, Free PMC Article
    3. The focal adhesion protein PINCH-1 associates with EPLIN at integrin adhesion sites. Karaköse E, et al. J Cell Sci, 2015 Mar 1. PMID 25609703, Free PMC Article
    4. Mechanosensitive EPLIN-dependent remodeling of adherens junctions regulates epithelial reshaping. Taguchi K, et al. J Cell Biol, 2011 Aug 22. PMID 21844208, Free PMC Article
    5. Extracellular signal-regulated kinase/mitogen-activated protein kinase regulates actin organization and cell motility by phosphorylating the actin cross-linking protein EPLIN. Han MY, et al. Mol Cell Biol, 2007 Dec. PMID 17875928, Free PMC Article

    See all (57) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Lipotoxic hepatocyte derived LIMA1 enriched small extracellular vesicles promote hepatic stellate cells activation via inhibiting mitophagy.
      Title: Lipotoxic hepatocyte derived LIMA1 enriched small extracellular vesicles promote hepatic stellate cells activation via inhibiting mitophagy.
    2. Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism.
      Title: Lima1 mediates the pluripotency control of membrane dynamics and cellular metabolism.
    3. this study identifies LIMA1 as a key protein regulating intestinal cholesterol absorption.
      Title: A LIMA1 variant promotes low plasma LDL cholesterol and decreases intestinal cholesterol absorption.
    4. findings suggest that PINCH-1 regulates integrin-mediated adhesion of keratinocytes through the interactions with ILK as well as EPLIN.
      Title: The focal adhesion protein PINCH-1 associates with EPLIN at integrin adhesion sites.
    5. Eplin-alpha transcription is regulated by actin-MAL-SRF signalling.
      Title: Epithelial Protein Lost in Neoplasm alpha (Eplin-alpha) is transcriptionally regulated by G-actin and MAL/MRTF coactivators.
    6. EPLIN functions to link the cadherin-catenin complex to F-actin and simultaneously stabilizes this population of actin fibers, resulting in the establishment of the adhesion
      Title: EPLIN mediates linkage of the cadherin catenin complex to F-actin and stabilizes the circumferential actin belt.
    7. ERK-mediated phosphorylation of EPLIN contributes to actin filament reorganization and enhanced cell motility.
      Title: Extracellular signal-regulated kinase/mitogen-activated protein kinase regulates actin organization and cell motility by phosphorylating the actin cross-linking protein EPLIN.
    8. EPLIN may function in growth control by associating with and regulating the actin cytoskeleton
      Title: Inhibition of anchorage-independent growth of transformed NIH3T3 cells by epithelial protein lost in neoplasm (EPLIN) requires localization of EPLIN to actin cytoskeleton.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (2) 
    • Gene trapped (1) 
    • Targeted (4)  1 citation

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • D15Ertd366e, 1110021C24Rik, 3526402A12Rik

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables actin filament binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables actin filament binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables actin filament binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables actin filament binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables actin monomer binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables actin monomer binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in actin filament bundle assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in actin filament bundle assembly ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in actin filament bundle assembly ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in cell migration IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cholesterol homeostasis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cholesterol metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in intestinal cholesterol absorption IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of actin filament depolymerization ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of actin filament depolymerization ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in ruffle organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ruffle organization ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in ruffle organization ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    Component Evidence Code Pubs
    is_active_in actin cytoskeleton IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in actin cytoskeleton IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in actin cytoskeleton ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in brush border IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in brush border membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cleavage furrow ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cleavage furrow ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in focal adhesion ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in focal adhesion ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in ruffle IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in stress fiber ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in stress fiber ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  

    General protein information

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    Preferred Names
    LIM domain and actin-binding protein 1
    Names
    epithelial protein lost in neoplasm

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001113545.2 → NP_001107017.1  LIM domain and actin-binding protein 1 isoform a

      See identical proteins and their annotated locations for NP_001107017.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (a) encodes the longer isoform (a).
      Source sequence(s)
      AC134548, AC138177
      Consensus CDS
      CCDS49729.1
      UniProtKB/Swiss-Prot
      Q8K2H0, Q9ERG0, Q9ERG1
      UniProtKB/TrEMBL
      Q8C7S2
      Related
      ENSMUSP00000073371.4, ENSMUST00000073691.5
      Conserved Domains (1) summary
      cd09485
      Location:388 → 440
      LIM_Eplin_alpha_beta; The Lim domain of Epithelial Protein Lost in Neoplasm (Eplin)

    2. NM_023063.5 → NP_075550.2  LIM domain and actin-binding protein 1 isoform b

      See identical proteins and their annotated locations for NP_075550.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (b) uses an alternate 5'-terminal exon, lacks part of the 5' coding region, and initiates translation from a downstream start codon, compared to variant a. The encoded isoform (b) has a shorter N-terminus, compared to isoform a.
      Source sequence(s)
      AC134548
      Consensus CDS
      CCDS37206.1
      UniProtKB/TrEMBL
      Q8BT15, Q8CD09
      Related
      ENSMUSP00000104652.3, ENSMUST00000109024.9
      Conserved Domains (1) summary
      cd09485
      Location:228 → 280
      LIM_Eplin_alpha_beta; The Lim domain of Epithelial Protein Lost in Neoplasm (Eplin)

    RNA

    1. NR_177985.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC134548, AC138177

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000081.7 Reference GRCm39 C57BL/6J

      Range
      99676351..99773292 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_006521245.2 → XP_006521308.1  LIM domain and actin-binding protein 1 isoform X1

      Conserved Domains (1) summary
      cd09485
      Location:101 → 153
      LIM_Eplin_alpha_beta; The Lim domain of Epithelial Protein Lost in Neoplasm (Eplin)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9ERG0.3 GenPept UniProtKB/Swiss-Prot:Q9ERG0

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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