Nucb2 nucleobindin 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Nucb2provided by RGD
Official Full Name: nucleobindin 2provided by RGD
Primary source: RGD:620888
See related: EnsemblRapid:ENSRNOG00000020456 AllianceGenome:RGD:620888
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: p54; Nefa
Summary: Enables G-protein alpha-subunit binding activity; guanyl-nucleotide exchange factor activity; and tumor necrosis factor receptor binding activity. Involved in several processes, including negative regulation of cell communication; positive regulation of testosterone secretion; and response to melanocyte-stimulating hormone. Located in Golgi medial cisterna and extracellular space. Biomarker of alcohol dependence; obesity; and type 2 diabetes mellitus. Orthologous to human NUCB2 (nucleobindin 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Lung (RPKM 302.2), Liver (RPKM 278.6) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q35

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (180185121..180221644)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (170750830..170787356)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (185106773..185143278, complement)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nesfatin-1 inhibits cerebral aneurysms by activating Nrf2 and inhibiting NF-kappaB signaling.
Title: Nesfatin-1 inhibits cerebral aneurysms by activating Nrf2 and inhibiting NF-κB signaling.
Nesfatin-1 alleviates hyperoxia-induced bronchopulmonary dysplasia (BPD) via the nuclear factor-kappaB (NF-kappaB) p65 signaling pathway.
Title: Nesfatin-1 alleviates hyperoxia-induced bronchopulmonary dysplasia (BPD) via the nuclear factor-κB (NF-κB) p65 signaling pathway.
Nuclear Binding Protein 2/Nesfatin-1 Affects Trophoblast Cell Fusion during Placental Development via the EGFR-PLCG1-CAMK4 Pathway.
Title: Nuclear Binding Protein 2/Nesfatin-1 Affects Trophoblast Cell Fusion during Placental Development via the EGFR-PLCG1-CAMK4 Pathway.
Nesfatin-1 peptide protects rat renal epithelial cells against high glucose and H2O2 induced injury via inhibition of oxidative stress, apoptosis, and fibrosis.
Title: Nesfatin-1 peptide protects rat renal epithelial cells against high glucose and H(2)O(2) induced injury via inhibition of oxidative stress, apoptosis, and fibrosis.
The role of serum nesfatin-1 in a rat model of acute pancreatitis.
Title: The role of serum nesfatin-1 in a rat model of acute pancreatitis.
A research on the expression of Nucb2/ cAMP / PKA in the hippocampus of alcohol-dependent rats.
Title: A research on the expression of Nucb2/ cAMP / PKA in the hippocampus of alcohol-dependent rats.
Nesfatin-1 attenuates injury in a rat model of myocardial infarction by targeting autophagy, inflammation, and apoptosis.
Title: Nesfatin-1 attenuates injury in a rat model of myocardial infarction by targeting autophagy, inflammation, and apoptosis.
Expression of phoenixin-14 and nesfatin-1 in the hypothalamo-pituitary-gonadal axis in the phases of the estrous cycle.
Title: Expression of phoenixin-14 and nesfatin-1 in the hypothalamo-pituitary-gonadal axis in the phases of the estrous cycle.
Knockdown of Endogenous Nucb2/Nesfatin-1 in the PVN Leads to Obese-Like Phenotype and Abolishes the Metformin- and Stress-Induced Thermogenic Response in Rats.
Title: Knockdown of Endogenous Nucb2/Nesfatin-1 in the PVN Leads to Obese-Like Phenotype and Abolishes the Metformin- and Stress-Induced Thermogenic Response in Rats.
Downregulation of nesfatin-1 expression in acute kidney injury in vivo in wistar rats and in vitro in cultured cells.
Title: Downregulation of nesfatin-1 expression in acute kidney injury in vivo in wistar rats and in vitro in cultured cells.

Submit: New GeneRIF Correction See all GeneRIFs (86)

General gene information

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Markers

GDB:177399 (e-PCR)
- UniSTS:99332

BI275463 (e-PCR)
- UniSTS:249636

D1Rat187 (e-PCR), detects polymorphism
- UniSTS:227052

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables G-protein alpha-subunit binding	IDA Inferred from Direct Assay more info	PubMed
enables calcium ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables guanyl-nucleotide exchange factor activity	IDA Inferred from Direct Assay more info	PubMed
enables tumor necrosis factor receptor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in insulin metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of appetite	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cAMP/PKA signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of fat cell differentiation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of glucagon secretion	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of insulin receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of fatty acid oxidation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of testosterone secretion	IDA Inferred from Direct Assay more info	PubMed
involved_in response to glucose	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to human chorionic gonadotropin	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to melanocyte-stimulating hormone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to starvation	IEP Inferred from Expression Pattern more info	PubMed
involved_in sequestering of metal ion	ISO Inferred from Sequence Orthology more info
involved_in small GTPase-mediated signal transduction	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in Golgi medial cisterna	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in endomembrane system	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
is_active_in endoplasmic reticulum-Golgi intermediate compartment	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum-Golgi intermediate compartment	ISO Inferred from Sequence Orthology more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in nuclear outer membrane	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: nucleobindin-2

Names: DNA-binding protein NEFA; nesfatin-1; prepronefastin; prepronesfatin

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_021663.2 → NP_067695.1 nucleobindin-2 precursor

See identical proteins and their annotated locations for NP_067695.1

Status: PROVISIONAL

Source sequence(s)

BC061778

UniProtKB/Swiss-Prot

Q9JI85

UniProtKB/TrEMBL

A6I8F1

Conserved Domains (3) summary

pfam13499
Location:247 → 321

EF-hand_7; EF-hand domain pair

pfam14235
Location:328 → 383

DUF4337; Domain of unknown function (DUF4337)

cl08302
Location:250 → 321

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

180185121..180221644

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006230095.5 → XP_006230157.1 nucleobindin-2 isoform X1

See identical proteins and their annotated locations for XP_006230157.1

UniProtKB/TrEMBL

A0A8I6GHJ0, A6I8F0, A6I8F1

Related

ENSRNOP00000027753.4, ENSRNOT00000027752.7

Conserved Domains (3) summary

pfam13499
Location:247 → 321

EF-hand_7; EF-hand domain pair

pfam14235
Location:328 → 383

DUF4337; Domain of unknown function (DUF4337)

cl08302
Location:250 → 321

EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...