Napepld N-acyl phosphatidylethanolamine phospholipase D [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Napepldprovided by RGD
Official Full Name: N-acyl phosphatidylethanolamine phospholipase Dprovided by RGD
Primary source: RGD:735197
See related: EnsemblRapid:ENSRNOG00000011363 AllianceGenome:RGD:735197
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: NAPE-PLD
Summary: Enables phospholipase activity. Involved in negative regulation of eating behavior; phospholipid metabolic process; and response to isolation stress. Predicted to be located in Golgi apparatus; early endosome; and nucleus. Predicted to be active in hippocampal mossy fiber to CA3 synapse; smooth endoplasmic reticulum membrane; and synaptic membrane. Biomarker of osteoarthritis. Orthologous to human NAPEPLD (N-acyl phosphatidylethanolamine phospholipase D). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Heart (RPKM 39.3), Kidney (RPKM 30.7) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 4q11

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	4	NC_086022.1 (14251591..14291010)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	4	NC_051339.1 (13360532..13398815)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	4	NC_005103.4 (9965323..10004650)

Chromosome 4 - NC_086022.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our results indicate that PGD(2) stimulatory effect involves both PLD and ERK1/2-MAPK activation, and both pathways operate independently of PC synthesis homeostasis.
Title: p44/42(ERK1/2) MAPK and PLD activation by PGD2 preserves papillary phosphatidylcholine homeostasis.
These results demonstrated wide distribution of NAPE-PLD in various brain regions and its age-dependent expression, suggesting the central role of this enzyme in the formation of anandamide and other N-acylethanolamines in the brain.
Title: Regional distribution and age-dependent expression of N-acylphosphatidylethanolamine-hydrolyzing phospholipase D in rat brain.

Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables N-acylphosphatidylethanolamine-specific phospholipase D activity	IBA Inferred from Biological aspect of Ancestor more info
enables N-acylphosphatidylethanolamine-specific phospholipase D activity	IEA Inferred from Electronic Annotation more info
enables N-acylphosphatidylethanolamine-specific phospholipase D activity	ISO Inferred from Sequence Orthology more info
enables N-acylphosphatidylethanolamine-specific phospholipase D activity	ISS Inferred from Sequence or Structural Similarity more info
enables bile acid binding	IEA Inferred from Electronic Annotation more info
enables bile acid binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables phospholipase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in N-acylethanolamine metabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within N-acylethanolamine metabolic process	ISO Inferred from Sequence Orthology more info
involved_in N-acylphosphatidylethanolamine metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in N-acylphosphatidylethanolamine metabolic process	IEA Inferred from Electronic Annotation more info
involved_in N-acylphosphatidylethanolamine metabolic process	ISO Inferred from Sequence Orthology more info
involved_in N-acylphosphatidylethanolamine metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in host-mediated regulation of intestinal microbiota composition	ISO Inferred from Sequence Orthology more info
involved_in host-mediated regulation of intestinal microbiota composition	ISS Inferred from Sequence or Structural Similarity more info
involved_in lipid catabolic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of eating behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipid catabolic process	IEA Inferred from Electronic Annotation more info
involved_in phospholipid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of brown fat cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of brown fat cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of inflammatory response	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to isolation stress	IEP Inferred from Expression Pattern more info	PubMed
involved_in temperature homeostasis	ISO Inferred from Sequence Orthology more info
involved_in temperature homeostasis	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in Golgi apparatus	ISS Inferred from Sequence or Structural Similarity more info
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in early endosome	ISS Inferred from Sequence or Structural Similarity more info
located_in early endosome membrane	IEA Inferred from Electronic Annotation more info
is_active_in hippocampal mossy fiber to CA3 synapse	ISO Inferred from Sequence Orthology more info
is_active_in membrane-bounded organelle	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear envelope	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleoplasm	ISS Inferred from Sequence or Structural Similarity more info
is_active_in postsynaptic membrane	ISO Inferred from Sequence Orthology more info
is_active_in presynapse	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic membrane	ISO Inferred from Sequence Orthology more info
is_active_in smooth endoplasmic reticulum membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D

Names: NAPE-hydrolyzing phospholipase D

NP_955413.1

EC 3.1.4.54

XP_006235970.1

EC 3.1.4.54

XP_006235972.1

EC 3.1.4.54

XP_038963172.1

EC 3.1.4.54

XP_063141777.1

EC 3.1.4.54

XP_063141778.1

EC 3.1.4.54

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_199381.2 → NP_955413.1 N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D

See identical proteins and their annotated locations for NP_955413.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000004

UniProtKB/Swiss-Prot

Q769K2

UniProtKB/TrEMBL

A0A8I6AKD5, A6K5B2

Conserved Domains (1) summary

cl23716
Location:129 → 316

metallo-hydrolase-like_MBL-fold; mainly hydrolytic enzymes and related proteins which carry out various biological functions; MBL-fold metallohydrolase domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086022.1 Reference GRCr8

Range

14251591..14291010

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006235908.5 → XP_006235970.1 N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform X1

UniProtKB/TrEMBL

A0A8I6AKD5

Related

ENSRNOP00000108050.1, ENSRNOT00000136190.1

Conserved Domains (2) summary

COG2220
Location:193 → 462

UlaG; L-ascorbate metabolism protein UlaG, beta-lactamase superfamily [Carbohydrate transport and metabolism]

cl23716
Location:202 → 389

metallo-hydrolase-like_MBL-fold; mainly hydrolytic enzymes and related proteins which carry out various biological functions; MBL-fold metallohydrolase domain
XM_039107244.2 → XP_038963172.1 N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform X3

UniProtKB/Swiss-Prot

Q769K2

UniProtKB/TrEMBL

A0A8I6AKD5, A6K5B2

Conserved Domains (1) summary

cl23716
Location:129 → 316

metallo-hydrolase-like_MBL-fold; mainly hydrolytic enzymes and related proteins which carry out various biological functions; MBL-fold metallohydrolase domain
XM_006235910.5 → XP_006235972.1 N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform X2

UniProtKB/TrEMBL

A0A0G2K5Y7, A0A8I6AKD5

Related

ENSRNOP00000073608.2, ENSRNOT00000086609.3

Conserved Domains (2) summary

COG2220
Location:128 → 397

UlaG; L-ascorbate metabolism protein UlaG, beta-lactamase superfamily [Carbohydrate transport and metabolism]

cl23716
Location:137 → 324

metallo-hydrolase-like_MBL-fold; mainly hydrolytic enzymes and related proteins which carry out various biological functions; MBL-fold metallohydrolase domain
XM_063285708.1 → XP_063141778.1 N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform X3

UniProtKB/Swiss-Prot

Q769K2

UniProtKB/TrEMBL

A6K5B2

Related

ENSRNOP00000015322.5, ENSRNOT00000015322.5
XM_063285707.1 → XP_063141777.1 N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D isoform X3

UniProtKB/Swiss-Prot

Q769K2

UniProtKB/TrEMBL

A6K5B2

Related

ENSRNOP00000082713.2, ENSRNOT00000104090.2