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    SSRP1 structure specific recognition protein 1 [ Homo sapiens (human) ]

    Gene ID: 6749, updated on 28-Oct-2024

    Summary

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    Official Symbol
    SSRP1provided by HGNC
    Official Full Name
    structure specific recognition protein 1provided by HGNC
    Primary source
    HGNC:HGNC:11327
    See related
    Ensembl:ENSG00000149136 MIM:604328; AllianceGenome:HGNC:11327
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    FACT; T160; FACT80
    Summary
    The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. [provided by RefSeq, Jul 2008]
    Expression
    Ubiquitous expression in lymph node (RPKM 33.5), ovary (RPKM 32.7) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See SSRP1 in Genome Data Viewer
    Location:
    11q12.1
    Exon count:
    17
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 11 NC_000011.10 (57325988..57335892, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 11 NC_060935.1 (57275479..57285383, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 11 NC_000011.9 (57093462..57103366, complement)

    Chromosome 11 - NC_000011.10Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC107984364 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr11:57051243-57052127 Neighboring gene MPRA-validated peak1278 silencer Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr11:57067393-57068592 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr11:57070511-57071048 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr11:57071049-57071584 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr11:57071585-57072121 Neighboring gene tankyrase 1 binding protein 1 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3343 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3344 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr11:57092719-57093592 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4714 Neighboring gene small nucleolar RNA U13 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4715 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3345 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4716 Neighboring gene purinergic receptor P2X 3 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr11:57117303-57117802 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr11:57132373-57132874 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr11:57136946-57138145 Neighboring gene BRD4-independent group 4 enhancer GRCh37_chr11:57146812-57148011 Neighboring gene proteoglycan 3, pro eosinophil major basic protein 2

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. Isolation and characterization of human cDNA clones encoding a high mobility group box protein that recognizes structural distortions to DNA caused by binding of the anticancer agent cisplatin. Bruhn SL, et al. Proc Natl Acad Sci U S A, 1992 Mar 15. PMID 1372440, Free PMC Article
    2. Crystal Structure of Human SSRP1 Middle Domain Reveals a Role in DNA Binding. Zhang W, et al. Sci Rep, 2015 Dec 21. PMID 26687053, Free PMC Article
    3. Integrated molecular mechanism directing nucleosome reorganization by human FACT. Tsunaka Y, et al. Genes Dev, 2016 Mar 15. PMID 26966247, Free PMC Article
    4. Histone Chaperone SSRP1 is Essential for Wnt Signaling Pathway Activity During Osteoblast Differentiation. Hossan T, et al. Stem Cells, 2016 May. PMID 27146025
    5. Anticancer drug candidate CBL0137, which inhibits histone chaperone FACT, is efficacious in preclinical orthotopic models of temozolomide-responsive and -resistant glioblastoma. Barone TA, et al. Neuro Oncol, 2017 Feb 1. PMID 27370399, Free PMC Article

    See all (290) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Hypoxia-inducible factor 1 recruits FACT and RNF20/40 to mediate histone ubiquitination and transcriptional activation of target genes.
      Title: Hypoxia-inducible factor 1 recruits FACT and RNF20/40 to mediate histone ubiquitination and transcriptional activation of target genes.
    2. Histone chaperone SSRP1 is required for apoptosis inhibition and mitochondrial function in HCC via transcriptional promotion of TRAP1.
      Title: Histone chaperone SSRP1 is required for apoptosis inhibition and mitochondrial function in HCC via transcriptional promotion of TRAP1.
    3. Human FACT subunits coordinate to catalyze both disassembly and reassembly of nucleosomes.
      Title: Human FACT subunits coordinate to catalyze both disassembly and reassembly of nucleosomes.
    4. Electron microscopy analysis of ATP-independent nucleosome unfolding by FACT.
      Title: Electron microscopy analysis of ATP-independent nucleosome unfolding by FACT.
    5. Dual targeting of the epigenome via FACT complex and histone deacetylase is a potent treatment strategy for DIPG.
      Title: Dual targeting of the epigenome via FACT complex and histone deacetylase is a potent treatment strategy for DIPG.
    6. Structural insights into multifunctionality of human FACT complex subunit hSSRP1.
      Title: Structural insights into multifunctionality of human FACT complex subunit hSSRP1.
    7. Histone chaperone FACT complex inhibitor CBL0137 interferes with DNA damage repair and enhances sensitivity of medulloblastoma to chemotherapy and radiation.
      Title: Histone chaperone FACT complex inhibitor CBL0137 interferes with DNA damage repair and enhances sensitivity of medulloblastoma to chemotherapy and radiation.
    8. SSRP1 Is a Prognostic Biomarker Correlated with CD8(+) T Cell Infiltration in Hepatocellular Carcinoma (HCC).
      Title: SSRP1 Is a Prognostic Biomarker Correlated with CD8(+) T Cell Infiltration in Hepatocellular Carcinoma (HCC).
    9. CPF impedes cell cycle re-entry of quiescent lung cancer cells through transcriptional suppression of FACT and c-MYC.
      Title: CPF impedes cell cycle re-entry of quiescent lung cancer cells through transcriptional suppression of FACT and c-MYC.
    10. The role of FACT in managing chromatin: disruption, assembly, or repair?
      Title: The role of FACT in managing chromatin: disruption, assembly, or repair?
    Submit: New GeneRIF Correction See all GeneRIFs (54)

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    HIV-1 interactions

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    Replication interactions

    Interaction Pubs
    HIV-1 infection requires SSRP1 as shown through shRNA-mediated knockdown of SSRP1 in human CD4+ T cells PubMed
    shRNA knockdown of SSRP1 increases HIV-1 LTR-driven gene expression in HEK293 cells transduced with VSV-G pseudo-typed HIV-1 NL4-3-Luc construct; i.e. HIV-1 is restricted by SSRP1 PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Tat tat The heterodimeric complex FACT proteins SPT16 and SSRP1 are recognized to interact with HIV-1 in Jurkat cell PubMed
    tat HIV-1 Tat activated transcription facilitates removal of histones H2A and H2B at the LTR, and that the FACT complex, at least consisting of SPT16 and SSRP1, may be responsible for their removal PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA binding HDA PubMed 
    enables histone binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables nucleosome binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in nucleosome assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in nucleosome disassembly IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of chromatin organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in regulation of chromatin organization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of FACT complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of FACT complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in nucleolus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    FACT complex subunit SSRP1
    Names
    FACT 80 kDa subunit
    FACTp80
    chromatin-specific transcription elongation factor 80 kDa subunit
    cisplatin-DNA SSRP
    facilitates chromatin remodeling 80 kDa subunit
    facilitates chromatin transcription complex 80 kDa subunit
    facilitates chromatin transcription complex subunit SSRP1
    hSSRP1
    high mobility group box
    recombination signal sequence recognition protein 1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_003146.3 → NP_003137.1  FACT complex subunit SSRP1

      See identical proteins and their annotated locations for NP_003137.1

      Status: REVIEWED

      Source sequence(s)
      AP000781
      Consensus CDS
      CCDS7952.1
      UniProtKB/Swiss-Prot
      Q08945, Q5BJG8
      Related
      ENSP00000278412.2, ENST00000278412.7
      Conserved Domains (2) summary
      COG5165
      Location:21 → 480
      POB3; Nucleosome-binding factor SPN, POB3 subunit [Transcription / DNA replication, recombination, and repair / Chromatin structure and dynamics]
      pfam00505
      Location:547 → 614
      HMG_box; HMG (high mobility group) box

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000011.10 Reference GRCh38.p14 Primary Assembly

      Range
      57325988..57335892 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047427498.1 → XP_047283454.1  FACT complex subunit SSRP1 isoform X2

      UniProtKB/Swiss-Prot
      Q08945, Q5BJG8

    2. XM_017018180.2 → XP_016873669.2  FACT complex subunit SSRP1 isoform X1

    3. XM_047427499.1 → XP_047283455.1  FACT complex subunit SSRP1 isoform X3

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060935.1 Alternate T2T-CHM13v2.0

      Range
      57275479..57285383 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054369757.1 → XP_054225732.1  FACT complex subunit SSRP1 isoform X2

      UniProtKB/Swiss-Prot
      Q08945, Q5BJG8

    2. XM_054369756.1 → XP_054225731.1  FACT complex subunit SSRP1 isoform X1

    3. XM_054369758.1 → XP_054225733.1  FACT complex subunit SSRP1 isoform X3

    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q08945.1 GenPept UniProtKB/Swiss-Prot:Q08945

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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