Figure 5. [A schematic representation of the...]. - Assay Guidance Manual

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Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-.

Assay Guidance Manual [Internet].

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Figure 5.

A schematic representation of the fluorogenic LDH-release assay chemistry. LDH from dead cells that leaked into the culture medium catalyzes the conversion of lactate to pyruvate and in the process generates NADH. In the presence of the diaphorase (reductase) enzyme, the NADH can reduce resazurin into the fluorogenic resorufin product that can be measured using a plate reading fluorometer.

From: Cytotoxicity Assays: In Vitro Methods to Measure Dead Cells

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Riss T, Niles A, Moravec R, et al. Cytotoxicity Assays: In Vitro Methods to Measure Dead Cells. 2019 May 1. In: Markossian S, Grossman A, Arkin M, et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-. Figure 5. [A schematic representation of the...].
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