Analysis of HeLa cells at 12 or 24 hours after transfection with wild type or mutant miR-1 or miR-124. miR-1 is preferentially expressed in heart and skeletal muscle, while miR-124 is preferentially expressed in brain. Results suggest that metazoan miRNAs can reduce the levels of target transcripts.
Lim LP, Lau NC, Garrett-Engele P, Grimson A et al. Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature 2005 Feb 17;433(7027):769-73. PMID: 15685193