Structural basis of transcription: alpha-amanitin-RNA polymerase II cocrystal at 2.8 A resolution

Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1218-22. doi: 10.1073/pnas.251664698. Epub 2002 Jan 22.

Abstract

The structure of RNA polymerase II in a complex with the inhibitor alpha-amanitin has been determined by x-ray crystallography. The structure of the complex indicates the likely basis of inhibition and gives unexpected insight into the transcription mechanism.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amanitins / chemistry*
  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Crystallography, X-Ray / methods
  • Hydrogen Bonding
  • Models, Molecular
  • Protein Conformation
  • Protein Structure, Secondary
  • RNA Polymerase II / chemistry*
  • Transcription, Genetic*

Substances

  • Amanitins
  • RNA Polymerase II

Associated data

  • PDB/1K83