Abstract
Transient interactions of platelet-receptor glycoprotein Ibalpha (GpIbalpha) and the plasma protein von Willebrand factor (VWF) reduce platelet velocity at sites of vascular damage and play a role in haemostasis and thrombosis. Here we present structures of the GpIbalpha amino-terminal domain and its complex with the VWF domain A1. In the complex, GpIbalpha wraps around one side of A1, providing two contact areas bridged by an area of solvated charge interaction. The structures explain the effects of gain-of-function mutations related to bleeding disorders and provide a model for shear-induced activation. These detailed insights into the initial interactions in platelet adhesion are relevant to the development of antithrombotic drugs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Substitution
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Bernard-Soulier Syndrome / genetics
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Bernard-Soulier Syndrome / metabolism
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Binding Sites
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Blood Platelets / metabolism
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Blood Platelets / physiology
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Crystallization
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Crystallography, X-Ray
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Humans
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Hydrogen Bonding
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Ligands
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Models, Molecular
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Mutation
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Platelet Adhesiveness
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Platelet Glycoprotein GPIb-IX Complex / chemistry*
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Platelet Glycoprotein GPIb-IX Complex / genetics
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Platelet Glycoprotein GPIb-IX Complex / metabolism*
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Protein Conformation
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Protein Structure, Quaternary
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Protein Structure, Secondary
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Protein Structure, Tertiary
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism
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Repetitive Sequences, Amino Acid
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Static Electricity
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von Willebrand Diseases / genetics
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von Willebrand Diseases / metabolism
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von Willebrand Factor / chemistry*
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von Willebrand Factor / metabolism*
Substances
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Ligands
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Platelet Glycoprotein GPIb-IX Complex
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Recombinant Proteins
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von Willebrand Factor