Abstract
Guanine nucleotide binding (G) protein levels and functioning in the platelets of 19 methadone-maintained patients were compared to age and sex matched, normal controls. We found that in the methadone patients, G alpha s-levels were significantly higher, while the levels of G alpha i 1/2 and pertussis toxin catalyzed [32P]ADP ribosylation were significantly lower compared to control subjects in platelet membranes. We have further found that when all three of these biochemical indicators were combined in a discriminant function analysis, 79% of the methadone patients were correctly classified and 83% of the controls were correctly classified.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Diphosphate Ribose / blood
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Adult
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Blood Platelets / drug effects
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Blood Platelets / metabolism
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Cell Membrane / metabolism
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Female
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GTP-Binding Protein alpha Subunits, Gi-Go / blood
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GTP-Binding Protein alpha Subunits, Gs / blood
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GTP-Binding Proteins / blood*
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Humans
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Male
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Methadone / therapeutic use*
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Opioid-Related Disorders / blood*
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Opioid-Related Disorders / diagnosis
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Opioid-Related Disorders / rehabilitation
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Reference Values
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Sensitivity and Specificity
Substances
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Adenosine Diphosphate Ribose
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GTP-Binding Proteins
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GTP-Binding Protein alpha Subunits, Gi-Go
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GTP-Binding Protein alpha Subunits, Gs
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Methadone