Lactase phlorhizin hydrolase turnover in vivo in water-fed and colostrum-fed newborn pigs

Biochem J. 1996 Dec 15;320 ( Pt 3)(Pt 3):735-43. doi: 10.1042/bj3200735.

Abstract

We have estimated the synthesis rates in vivo of precursor and brush-border (BB) polypeptides of lactase phlorhizin hydrolase (LPH) in newborn pigs fed with water or colostrum for 24h post partum. At the end of the feeding period, piglets were anaesthetized and infused intravenously for 3h with L-[4-3H]- phenylalanine. Blood and jejunal samples were collected at timed intervals. The precursor and BB forms of LPH were isolated from jejunal mucosa by immunoprecipitation followed by SDS/PAGE, and their specific radioactivity in Phe determined. The kinetics of precursor and BB LPH labelling were analysed by using a linear compartmental model. Immunoisolated LPH protein consisted of five polypeptides [high-mannose LPH precursor (proLPHh), complex glycosylated LPH precursor (proLPHe), intermediate complex glycosylated LPH precursor (proLPH1i) and two forms of BB LPH]. The fractional synthesis rate (Ks) of proLPHh and proLPHc (approx. 5%/min) were the same in the two groups but the absolute synthesis rate (in arbitrary units, min-1) of proLPHh in the colostrum-fed animals was twice that of the water-fed animals. The Ks values of proLPHi polypeptides were significantly different (water-fed, 3.89%/min; colostrum-fed, 1.6%/min), but the absolute synthesis rates did not differ. The Ks of BB LPH was not different between experimental treatment groups (on average 0.037%/min). However, the proportion of newly synthesized proLPHh processed to BB LPH was 48% lower in colostrum-fed than in water-fed animals. We conclude that in neonatal pigs, the ingestion of colostrum stimulates the synthesis of proLPHh but, at least temporarily, disrupts the processing of proLPH polypeptides to the BB enzyme.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Amino Acids / blood
  • Animals
  • Colostrum / metabolism*
  • Diet
  • Electrophoresis, Polyacrylamide Gel
  • Glycoside Hydrolases / metabolism
  • Intestinal Mucosa / chemistry
  • Intestinal Mucosa / enzymology
  • Intestinal Mucosa / metabolism
  • Kinetics
  • Lactase-Phlorizin Hydrolase / biosynthesis
  • Lactase-Phlorizin Hydrolase / chemistry
  • Lactase-Phlorizin Hydrolase / metabolism*
  • Models, Biological
  • Molecular Sequence Data
  • Peptide Biosynthesis
  • Peptides / chemistry
  • Peptides / isolation & purification
  • Precipitin Tests
  • Protein Processing, Post-Translational / physiology
  • Sequence Analysis
  • Swine
  • Water / metabolism

Substances

  • Amino Acids
  • Peptides
  • Water
  • Glycoside Hydrolases
  • Lactase-Phlorizin Hydrolase