Abstract
Immunomagnetic separation has been used to enrich CD34-positive cells in umbilical cord blood. Cell purities were increased from 0.59% preseparation to 92.7% postseparation (n = 16) with a mean yield of 75.7%. CFU were enriched 127 fold by immunomagnetic separation. Addition of combinations of recombinant growth factors resulted in cloning efficiencies of greater than 50%.
MeSH terms
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, CD34
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Cell Count
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Colony-Forming Units Assay
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Fetal Blood / cytology*
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Hematopoietic Cell Growth Factors / pharmacology
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Hematopoietic Stem Cells* / drug effects
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Humans
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Immunomagnetic Separation* / instrumentation
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Immunophenotyping
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Infant, Newborn
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Stem Cell Factor
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, CD34
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Hematopoietic Cell Growth Factors
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Stem Cell Factor