Complete Genome Sequence of the Multidrug-Resistant Pseudomonas aeruginosa Endemic Houston-1 Strain, Isolated from a Pediatric Patient with Cystic Fibrosis and Assembled Using Oxford Nanopore and Illumina Sequencing

Jennifer K Spinler; Sabeen Raza; Jessica K Runge; Ruth Ann Luna

doi:10.1128/MRA.00903-19

Complete Genome Sequence of the Multidrug-Resistant Pseudomonas aeruginosa Endemic Houston-1 Strain, Isolated from a Pediatric Patient with Cystic Fibrosis and Assembled Using Oxford Nanopore and Illumina Sequencing

Microbiol Resour Announc. 2019 Oct 24;8(43):e00903-19. doi: 10.1128/MRA.00903-19.

Authors

Jennifer K Spinler^{1

2}, Sabeen Raza^{3

2}, Jessica K Runge^{3

2}, Ruth Ann Luna^{3

2}

Affiliations

¹ Department of Pathology & Immunology, Baylor College of Medicine, Houston, Texas, USA spinler@bcm.edu.
² Texas Children's Microbiome Center, Department of Pathology, Texas Children's Hospital, Houston, Texas, USA.
³ Department of Pathology & Immunology, Baylor College of Medicine, Houston, Texas, USA.

Abstract

Hybrid de novo assembly of Illumina/Nanopore sequence data produced complete circular sequences of the chromosome and a plasmid for the multidrug-resistant Pseudomonas aeruginosa Houston-1 strain. This provides a high-quality representative sequence for a lineage endemic to a pediatric cystic fibrosis care center at Texas Children's Hospital.