Comparison of Microneutralization and Histo-Blood Group Antigen-Blocking Assays for Functional Norovirus Antibody Detection

J Infect Dis. 2020 Feb 18;221(5):739-743. doi: 10.1093/infdis/jiz526.

Abstract

Background: The development of an in vitro cultivation system for human noroviruses allows the measurement of neutralizing antibody levels.

Methods: Serum neutralizing antibody levels were determined using a GII.4/Sydney/2012-like virus in human intestinal enteroids in samples collected before and 4 weeks after administration of an investigational norovirus vaccine and were compared with those measured in histo-blood group antigen (HBGA)-blocking assays.

Results: Neutralizing antibody seroresponses were observed in 71% of 24 vaccinated adults, and antibody levels were highly correlated (r = 0.82, P < .001) with those measured by HBGA blocking.

Conclusions: HBGA-blocking antibodies are a surrogate for neutralization in human noroviruses.

Clinical trials registration: NCT02475278.

Keywords: human norovirus; antibody; histo-blood group antigen; human intestinal enteroids; immunogenicity; neutralization; vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antibodies, Blocking / immunology*
  • Antibodies, Neutralizing / immunology*
  • Antibodies, Viral / analysis
  • Antibodies, Viral / immunology*
  • Blood Group Antigens / immunology*
  • Caliciviridae Infections / prevention & control*
  • Caliciviridae Infections / virology
  • Female
  • Healthy Volunteers
  • Humans
  • Male
  • Middle Aged
  • Neutralization Tests
  • Norovirus / immunology*
  • Vaccination*
  • Viral Vaccines / immunology*
  • Young Adult

Substances

  • Antibodies, Blocking
  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Blood Group Antigens
  • Viral Vaccines

Associated data

  • ClinicalTrials.gov/NCT02475278