Abstract
We have cloned a gene (BCY1) from the yeast Saccharomyces cerevisiae that encodes a regulatory subunit of the cyclic AMP-dependent protein kinase. The encoded protein has a structural organization similar to that of the RI and RII regulatory subunits of the mammalian cyclic AMP-dependent protein kinase. Strains of S. cerevisiae with disrupted BCY1 genes do not display a cyclic AMP-dependent protein kinase in vitro, fail to grow on many carbon sources, and are exquisitely sensitive to heat shock and starvation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Carrier Proteins / genetics*
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Cloning, Molecular*
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DNA Restriction Enzymes
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Genes*
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Genes, Fungal*
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Genetic Complementation Test
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Genetic Linkage
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Genotype
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Intracellular Signaling Peptides and Proteins*
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Macromolecular Substances
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Protein Kinases / genetics*
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
Substances
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Carrier Proteins
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Intracellular Signaling Peptides and Proteins
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Macromolecular Substances
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protein kinase modulator
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Protein Kinases
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DNA Restriction Enzymes