Chlorella viruses contain linear nonpermuted double-stranded DNA genomes with covalently closed hairpin ends

J Rohozinski; L E Girton; J L Van Etten

doi:10.1016/0042-6822(89)90277-8

Chlorella viruses contain linear nonpermuted double-stranded DNA genomes with covalently closed hairpin ends

Virology. 1989 Feb;168(2):363-9. doi: 10.1016/0042-6822(89)90277-8.

Authors

J Rohozinski¹, L E Girton, J L Van Etten

Affiliation

¹ Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.

PMID: 2916329
DOI: 10.1016/0042-6822(89)90277-8

Abstract

Pulsed field electrophoresis established that Chlorella viruses contain linear, nonpermuted, 330- to 380-kb dsDNA genomes. Terminal DNA restriction fragments of one virus, PBCV-1, were identified by Bal31 exonuclease digestion; the termini probably contain covalently closed hairpin ends. The end fragments cross-hybridize indicating terminal repetition; the region of repetition extends no more than 2.5 kb from the ends.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Chlorella*
DNA, Viral / analysis
DNA, Viral / genetics*
Genes, Viral*
Nucleic Acid Conformation
Nucleic Acid Hybridization
Restriction Mapping
Viruses / genetics*

Substances

DNA, Viral

Grants and funding

GM32441/GM/NIGMS NIH HHS/United States