A preliminary evaluation of glial cell line-derived neurotrophic factor (GDNF) levels in cerebrospinal fluid across various gestational ages and clinical conditions of the neonate

Rahul Rajkumar; Bhavana Bhaya; Divya Mamilla; Theresa Czech; Esther Kisseih; Arun Saini; Nitin Chouthai

doi:10.1016/j.ijdevneu.2017.10.001

A preliminary evaluation of glial cell line-derived neurotrophic factor (GDNF) levels in cerebrospinal fluid across various gestational ages and clinical conditions of the neonate

Int J Dev Neurosci. 2018 Apr:65:61-65. doi: 10.1016/j.ijdevneu.2017.10.001. Epub 2017 Oct 12.

Authors

Rahul Rajkumar¹, Bhavana Bhaya², Divya Mamilla³, Theresa Czech⁴, Esther Kisseih³, Arun Saini⁵, Nitin Chouthai⁶

Affiliations

¹ Department of Pediatrics, Division of Neonatal-Perinatal Medicine, Wayne State University, Detroit, MI, United States; Bloomberg School of Public Health, Department of International Health, Johns Hopkins University, Baltimore, MD, United States.
² Department of Pediatrics, Division of Neonatal-Perinatal Medicine, Wayne State University, Detroit, MI, United States; University Medical Center, Department of Internal Medicine, University of Nevada-Las Vegas, Las Vegas, NV, United States.
³ Department of Pediatrics, Division of Neonatal-Perinatal Medicine, Wayne State University, Detroit, MI, United States.
⁴ Department of Pediatrics, Division of Neonatal-Perinatal Medicine, Wayne State University, Detroit, MI, United States; Ann and Robert H. Lurie Children's Hospital, Department of Pediatrics, Division of Pediatric Neurology, Northwestern University, Chicago, IL, United States.
⁵ Department of Pediatrics, Division of Neonatal-Perinatal Medicine, Wayne State University, Detroit, MI, United States; Le Bonheur Children's Hospital, Department of Pediatrics, Division of Critical Care, University of Tennessee, Memphis, TN, United States.
⁶ Department of Pediatrics, Division of Neonatal-Perinatal Medicine, Wayne State University, Detroit, MI, United States. Electronic address: nchoutha@med.wayne.edu.

PMID: 29031644
DOI: 10.1016/j.ijdevneu.2017.10.001

Abstract

Background: This study aims to investigate glial cell derived neurotrophic factor (GDNF) levels in newborns' umbilical cord blood and cerebrospinal fluid across various perinatal growth parameters and clinical conditions.

Methods: Cord blood from 20 newborns and 58 residual CSF samples (stored after completion of clinical testing) were collected. GDNF levels were determined using GDNF ELISA kits from R&D Systems in triplicates with appropriate controls to eliminate background.

Results: Cord blood GDNF levels were significantly higher (p=0.004) in preterm newborns (n=6) (115.05±57.17,pg/ml) when compared to term newborns (n=14) (19.67±10.67,pg/ml). GDNF levels in CSF trended (p=0.07) higher in term newborns (n=10) (19.56±9.11,pg/ml) when compared to preterm newborns at term or post term corrected gestational ages (n=5) (14.49±3.53,pg/ml).

Conclusions: GDNF levels in preterm newborns were higher in cord blood and lower in CSF as compared to term newborns. It is important to further study circulating and CSF-GDNF levels in newborns at different gestational ages and clinical conditions.

Keywords: Cerebrospinal fluid (CSF); Gestational age (GA); Glial cell derived neurotrophic factor (GDNF); Intraventricular hemorrhage (IVH); Prematurity.

MeSH terms

Age Factors
Aging, Premature / cerebrospinal fluid*
Enzyme-Linked Immunosorbent Assay
Female
Fetal Blood / metabolism*
Gestational Age*
Glial Cell Line-Derived Neurotrophic Factor / blood*
Glial Cell Line-Derived Neurotrophic Factor / cerebrospinal fluid*
Humans
Infant, Newborn
Male
Pregnancy

Substances

Glial Cell Line-Derived Neurotrophic Factor