Abstract
Two regions of the Epstein-Barr virus BZLF1 trans-activator protein have sequence similarity to the c-fos protein. Part of the similarity corresponds to the region of c-fos which is similar to the DNA binding domain of c-jun and GCN-4. The structure of the exon which contains this region in c-fos and BZLF1 is also highly conserved between the two genes. Complete BZLF1 protein and a C terminal fragment were prepared either as purified fusion proteins or by in vitro translation from a BZLF1 cDNA. Gel retardation and DNase footprinting assays using these proteins show that BZLF1 is a sequence specific DNA binding protein capable of binding to a target sequence which contains a consensus AP-1 site.
MeSH terms
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Amino Acid Sequence
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Antigens / genetics
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Binding Sites
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Fungal Proteins / genetics
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Gene Products, tat
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Herpesvirus 4, Human / genetics
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Herpesvirus 4, Human / metabolism*
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Molecular Sequence Data
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Protein Kinases*
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins c-fos
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Proto-Oncogene Proteins c-jun
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Nucleic Acid
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Viral Proteins / genetics
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Viral Proteins / metabolism*
Substances
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Antigens
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DNA-Binding Proteins
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Fungal Proteins
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Gene Products, tat
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-fos
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Proto-Oncogene Proteins c-jun
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Viral Proteins
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Protein Kinases