The Trypanosoma brucei telomerase RNA (TER) homologue binds core proteins of the C/D snoRNA family

Sachin Kumar Gupta; Liat Kolet; Tirza Doniger; Viplov Kumar Biswas; Ron Unger; Yehuda Tzfati; Shulamit Michaeli

doi:10.1016/j.febslet.2013.03.017

The Trypanosoma brucei telomerase RNA (TER) homologue binds core proteins of the C/D snoRNA family

FEBS Lett. 2013 May 2;587(9):1399-404. doi: 10.1016/j.febslet.2013.03.017. Epub 2013 Mar 21.

Authors

Sachin Kumar Gupta¹, Liat Kolet, Tirza Doniger, Viplov Kumar Biswas, Ron Unger, Yehuda Tzfati, Shulamit Michaeli

Affiliation

¹ The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel.

PMID: 23523918
DOI: 10.1016/j.febslet.2013.03.017

Abstract

Trypanosome protozoan parasites are the causative agents of devastating diseases. Trypanosome telomeres grow in an uncontrolled manner and the variant surface glycoprotein (VSG) genes are located in subtelomeric domains. The gene encoding telomerase reverse transcriptase (TERT) was identified and in this study, we describe the Trypanosoma brucei telomerase RNA (TER). TER RNA is bound by the core proteins of the C/D small nucleolar RNA (snoRNA) family and associates with the methyltransferase-associated protein (MTAP), whose homologue binds to mammalian TER. Silencing of TbTER resulted in telomere shortening. This is the first report of a TER that binds the C/D snoRNA core proteins.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Base Sequence
Molecular Sequence Data
Protein Binding
RNA / genetics*
RNA / metabolism*
RNA, Small Nucleolar / genetics*
RNA, Small Nucleolar / metabolism*
Ribonucleoproteins, Small Nucleolar / metabolism*
Sequence Homology, Nucleic Acid*
Telomerase / genetics*
Telomerase / metabolism*
Trypanosoma brucei brucei / enzymology*
Trypanosoma brucei brucei / genetics

Substances

RNA, Small Nucleolar
Ribonucleoproteins, Small Nucleolar
telomerase RNA
RNA
Telomerase