Abstract
Transcriptional profiling methods have been utilized in the analysis of various biological processes in Dictyostelium. Recent advances in high-throughput sequencing have increased the resolution and the dynamic range of transcriptional profiling. Here we describe the utility of RNA sequencing with the Illumina technology for production of transcriptional profiles. We also describe methods for data mapping and storage as well as common and specialized tools for data analysis, both online and offline.
MeSH terms
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Base Sequence
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DNA Primers
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DNA, Complementary / genetics
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Data Mining
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Dictyostelium / genetics*
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Dictyostelium / metabolism
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Gene Expression Profiling / methods*
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Gene Library
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Genome, Protozoan
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High-Throughput Nucleotide Sequencing / methods
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Molecular Sequence Annotation
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Molecular Sequence Data
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Polymerase Chain Reaction
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Protozoan Proteins / genetics
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Protozoan Proteins / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / isolation & purification
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RNA, Messenger / metabolism
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RNA, Protozoan / genetics
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RNA, Protozoan / isolation & purification
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RNA, Protozoan / metabolism
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Sequence Analysis, RNA / methods*
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Software
Substances
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DNA Primers
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DNA, Complementary
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Protozoan Proteins
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RNA, Messenger
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RNA, Protozoan