Effect of sinefungin on macromolecular biosynthesis and cell cycle of Plasmodium falciparum

Trop Med Parasitol. 1990 Sep;41(3):273-8.

Abstract

The growth of the malarial parasite P. falciparum was arrested by the adenine containing nucleoside sinefungin at the trophozoite stage. The synthesis of DNA, of polyamines and the specific proteins of the schizont stage were completely blocked by the drug. The inhibition of DNA synthesis was not due to a decrease in the amount of DNA polymerase, but to the depletion of polyamines which are required for DNA synthesis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine / analogs & derivatives*
  • Adenosine / pharmacology
  • Adenosylmethionine Decarboxylase / antagonists & inhibitors
  • Animals
  • Autoradiography
  • DNA, Protozoan / biosynthesis*
  • DNA, Protozoan / drug effects
  • Electrophoresis, Polyacrylamide Gel
  • Molecular Structure
  • Plasmodium falciparum / cytology
  • Plasmodium falciparum / drug effects*
  • Plasmodium falciparum / metabolism
  • Polyamines / metabolism*
  • Protozoan Proteins / biosynthesis*
  • Protozoan Proteins / drug effects
  • Spermidine / biosynthesis
  • Spermine / biosynthesis

Substances

  • DNA, Protozoan
  • Polyamines
  • Protozoan Proteins
  • Spermine
  • Adenosylmethionine Decarboxylase
  • Adenosine
  • Spermidine
  • sinefungin