A plaque assay for the simian rotavirus SAII

J Gen Virol. 1979 Jun;43(3):513-9. doi: 10.1099/0022-1317-43-3-513.

Abstract

A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SAII is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SAII produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.

MeSH terms

  • Animals
  • Cell Line
  • DEAE-Dextran / pharmacology
  • Haplorhini
  • Kidney
  • Pancreatin / pharmacology
  • RNA Viruses / isolation & purification*
  • Rotavirus / drug effects
  • Rotavirus / isolation & purification*
  • Swine
  • Trypsin / pharmacology
  • Viral Plaque Assay / methods*
  • Virus Replication / drug effects

Substances

  • Pancreatin
  • DEAE-Dextran
  • Trypsin