Structure and function of the Epstein-Barr virus BZLF1 protein

G Packham; A Economou; C M Rooney; D T Rowe; P J Farrell

doi:10.1128/JVI.64.5.2110-2116.1990

Structure and function of the Epstein-Barr virus BZLF1 protein

J Virol. 1990 May;64(5):2110-6. doi: 10.1128/JVI.64.5.2110-2116.1990.

Authors

G Packham¹, A Economou, C M Rooney, D T Rowe, P J Farrell

Affiliation

¹ Ludwig Institute for Cancer Research, St. Mary's Hospital Medical School, London, England.

Abstract

Five DNA-binding sites for the Epstein-Barr virus BZLF1 protein have been identified within three of the early viral promoters, and four of these binding sites contain a consensus AP-1 site. The part of the BZLF1 protein required for sequence-specific DNA binding to one of these AP-1-like sites was identified by deletion mapping. Site-directed mutagenesis of this DNA target suggests that BZLF1 may work partly by overcoming a cellular repressor of viral transcription.

Publication types

Comparative Study

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cell Line
Chromosome Deletion
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / metabolism
Genes, Viral
Herpesvirus 4, Human / genetics*
Herpesvirus 4, Human / metabolism
Molecular Sequence Data
Mutation
Nucleotide Mapping
Oligonucleotide Probes
Plasmids
Promoter Regions, Genetic
Protein Biosynthesis
Restriction Mapping
Sequence Homology, Nucleic Acid
Trans-Activators*
Transcription, Genetic
Transfection
Viral Proteins / genetics*
Viral Proteins / metabolism

Substances

BZLF1 protein, Herpesvirus 4, Human
DNA-Binding Proteins
Oligonucleotide Probes
Trans-Activators
Viral Proteins