Abstract
In a single quantitative study, we measured acrA, acrB, tolC, mdfA, and norE expression in Escherichia coli clinical isolates by using real-time PCR. acrA and acrB overexpression strongly correlated with fluoroquinolone and multidrug resistance; tolC, mdfA, and norE expression did not. The order of abundance of efflux pump transcripts in all fluoroquinolone-susceptible isolates was tolC (highest), then acrA and acrB, and then mdfA and norE. Our findings suggest acrAB overexpression is an indicator of multidrug resistance.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Outer Membrane Proteins / metabolism
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Drug Resistance, Multiple, Bacterial / genetics*
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Escherichia coli / drug effects*
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Escherichia coli / genetics
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Escherichia coli / isolation & purification
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Escherichia coli / metabolism
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Fluoroquinolones / pharmacology*
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Gene Expression Regulation, Bacterial*
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Humans
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Lipoproteins / genetics
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Lipoproteins / metabolism
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / metabolism
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Microbial Sensitivity Tests
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Multidrug Resistance-Associated Proteins / genetics
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Multidrug Resistance-Associated Proteins / metabolism
Substances
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AcrA protein, E coli
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AcrB protein, E coli
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Anti-Bacterial Agents
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Bacterial Outer Membrane Proteins
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Escherichia coli Proteins
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Fluoroquinolones
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Lipoproteins
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Mdfa protein, E coli
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Membrane Transport Proteins
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Multidrug Resistance-Associated Proteins
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tolC protein, E coli