Abstract
The Nikon C1 confocal laser scanning microscope is a relatively inexpensive and user-friendly instrument. We describe a straightforward method to convert the C1 for multiphoton microscopy utilizing direct coupling of a femtosecond near-infrared laser into the scan head and fiber optic transmission of emission light to the three-channel detector box. Our adapted system can be rapidly switched between confocal and multiphoton mode, requires no modification to the original system, and uses only a few custom-made parts. The entire system, including scan mirrors and detector box, remain under the control of the user-friendly Nikon EZ-C1 software without modification.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Equipment Design
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Green Fluorescent Proteins / biosynthesis
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Green Fluorescent Proteins / chemistry
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Green Fluorescent Proteins / genetics
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Humans
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Image Processing, Computer-Assisted / instrumentation
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Image Processing, Computer-Assisted / methods
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Mice
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Mice, Transgenic
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Microscopy, Confocal / instrumentation*
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Microscopy, Confocal / methods
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Microscopy, Fluorescence, Multiphoton / instrumentation*
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Microscopy, Fluorescence, Multiphoton / methods
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Neurons
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Optical Fibers
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Pollen / ultrastructure
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Retina / cytology
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Retina / metabolism
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Rhodopsin / biosynthesis
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Rhodopsin / chemistry
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Rhodopsin / genetics
Substances
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Recombinant Fusion Proteins
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Green Fluorescent Proteins
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Rhodopsin