We present histologic findings of the aortic homograft mounted in a woven Dacron (C.R. Bard, Haverhill, PA) tube, which had been in the tricuspid position for 68 months in a patient with Ebstein anomaly. Although the layered architecture of leaflets was relatively well preserved, prominent calcification was observed in the sinus wall that had been in direct contact with the surrounding fabric. This sinus stiffness might have contributed to worsening of the valvar function.