Efficacy of grape seed proanthocyanidins on cardioprotection during isoproterenol-induced myocardial injury in rats

J Cardiovasc Pharmacol. 2009 Feb;53(2):109-15. doi: 10.1097/FJC.0b013e3181970c01.

Abstract

The present study was done to evaluate the role of grape seed proanthocyanidins (GSPs) in isoproterenol (ISO)-induced myocardial injury in rats. Male albino Wistar rats were pretreated with GSP (50, 100, and 150 mg/kg), 6 days a week, for 5 weeks. Induction of rats with ISO (85 mg/kg body weight, intraperitoneally) for 2 days resulted in a significant elevation of thiobarbituric acid-reactive substances in serum, mitochondrial cholesterol, triglycerides, and free fatty acids. A significant decrease was observed in serum reduced glutathione; ascorbic acid; alpha-tocopherol; ceruloplasmin; and mitochondrial cytochromes (b, c, c1, and aa3), phospholipids, and adenosine triphosphate. Pretreatment with GSP (100 and 150 mg/kg) positively altered the levels of all the parameters studied and restored normal mitochondrial function when compared with ISO-induced rats. The effect at a dose of 50 mg/kg was not promising when compared with the other 2 doses (100 and 150 mg/kg). These results confirm the efficacy of GSP in alleviating ISO-induced myocardial injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • Antioxidants / metabolism
  • Biomarkers / blood
  • Blood Chemical Analysis
  • Cardiotonic Agents / pharmacology
  • Cardiotonic Agents / therapeutic use*
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Grape Seed Extract
  • Isoproterenol
  • Lipid Metabolism / drug effects*
  • Male
  • Myocardial Ischemia / chemically induced
  • Myocardial Ischemia / drug therapy*
  • Myocardial Ischemia / metabolism
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Proanthocyanidins / pharmacology
  • Proanthocyanidins / therapeutic use*
  • Rats
  • Rats, Wistar
  • Thiobarbituric Acid Reactive Substances / metabolism

Substances

  • Antioxidants
  • Biomarkers
  • Cardiotonic Agents
  • Grape Seed Extract
  • Grape Seed Proanthocyanidins
  • Plant Extracts
  • Proanthocyanidins
  • Thiobarbituric Acid Reactive Substances
  • Isoproterenol