Abstract
Here we describe a knock-in mouse model for Cre-loxP-based conditional expression of TRPV1 in central nervous system neurons. Expression of Cre recombinase using biolistics, lentivirus or genetic intercrosses triggered heterologous expression of TRPV1 in a cell-specific manner. Application of the TRPV1 ligand capsaicin induced strong inward currents, triggered action potentials and activated stereotyped behaviors, allowing cell type-specific chemical genetic control of neuronal activity in vitro and in vivo.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Action Potentials / drug effects
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Action Potentials / genetics
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Animals
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Behavior, Animal* / drug effects
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Brain / metabolism
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Capsaicin / pharmacology
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Gene Targeting
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Genes, Reporter
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Genetic Vectors
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Green Fluorescent Proteins / genetics
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Immunohistochemistry
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Integrases* / biosynthesis
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Lentivirus / genetics
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Mice
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Mice, Knockout
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Neurons* / enzymology
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Neurons* / metabolism
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Neurons* / physiology
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Patch-Clamp Techniques
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Rats
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Recombination, Genetic*
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TRPV Cation Channels / genetics*
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Transfection
Substances
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TRPV Cation Channels
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Trpv1 protein, rat
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enhanced green fluorescent protein
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Green Fluorescent Proteins
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Cre recombinase
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Integrases
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Capsaicin