Lactadherin mediates sickle cell adhesion to vascular endothelial cells in flowing blood

Prasenjit Guchhait; Swapan K Dasgupta; Anhquyen Le; Sarvari Yellapragada; José A López; Perumal Thiagarajan

doi:10.3324/haematol.11379

Lactadherin mediates sickle cell adhesion to vascular endothelial cells in flowing blood

Haematologica. 2007 Sep;92(9):1266-7. doi: 10.3324/haematol.11379.

Authors

Prasenjit Guchhait, Swapan K Dasgupta, Anhquyen Le, Sarvari Yellapragada, José A López, Perumal Thiagarajan

PMID: 17768123
DOI: 10.3324/haematol.11379

Abstract

Increased exposure of sickle red blood cells to phosphatidylserine promotes its adhesion to the endothelium. A monoclonal antibody to lactadherin, a phosphatidylserine binding protein, inhibits sickle cell adhesion to histamine-stimulated endothelial cells in flowing blood. Added lactadherin enhances the adhesion via the integrin alphaVbeta3. These results indicate that lactadherin can mediate phosphatidylserine-expressing sickle cell adhesion to the endothelium.

Publication types

Letter
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Anemia, Sickle Cell / blood*
Animals
Antibodies, Monoclonal / pharmacology
Cattle
Cell Adhesion*
Endothelium, Vascular / metabolism*
Enzyme-Linked Immunosorbent Assay
Erythrocytes / cytology
Erythrocytes / metabolism
Erythrocytes, Abnormal / metabolism*
Humans
Integrin alphaVbeta3 / metabolism
Mice
Phagocytosis / physiology*
Phosphatidylserines / genetics
Phosphatidylserines / metabolism*
Protein Binding

Substances

Antibodies, Monoclonal
Integrin alphaVbeta3
Phosphatidylserines