Abstract
Signaling lymphocytic activation molecule (SLAM, CD150) is the universal morbillivirus receptor. Based on the identification of measles virus (MV) hemagglutinin (H) amino acids supporting human SLAM-dependent cell entry, we mutated canine distemper virus (CDV) H and identified residues necessary for efficient canine SLAM-dependent membrane fusion. These residues are located in two nearby clusters in a new CDV H structural model. To completely abolish SLAM-dependent fusion, combinations of mutations were necessary. We rescued a SLAM-blind recombinant CDV with six mutations that did not infect ferret peripheral blood mononuclear cells while retaining full infectivity in epithelial cells.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, CD
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Cells, Cultured
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Distemper Virus, Canine / physiology*
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Ferrets
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Glycoproteins*
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Hemagglutinins, Viral / genetics
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Hemagglutinins, Viral / physiology*
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Immunoglobulins*
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Leukocytes, Mononuclear / virology
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Membrane Fusion
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Models, Molecular
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Molecular Sequence Data
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Receptors, Cell Surface
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Receptors, Virus*
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Signaling Lymphocytic Activation Molecule Family Member 1
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Virus Replication
Substances
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Antigens, CD
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Glycoproteins
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Hemagglutinins, Viral
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Immunoglobulins
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Receptors, Cell Surface
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Receptors, Virus
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SLAMF1 protein, human
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Signaling Lymphocytic Activation Molecule Family Member 1