Abstract
Fifty-three southern USA Borrelia isolates were characterized using randomly amplified polymorphic DNA fingerprinting analysis (RAPD). Twenty-nine types were recognized among 37 B. andersonii strains, seven types among eight B. bissettii strains, and seven types among seven B. burgdorferi sensu stricto strains. Strain TXW-1 formed a separate RAPD type. Nearly complete sequences of the rrs genes from 17 representative southern Borrelia were determined. The similarity values were found to be 96-100% within the B. burgdorferi sensu lato (s.l.) complex, 94-99% among the relapsing fever borreliae, and 93-99% between the two complexes. Phylogenetic analysis indicated that all the Borrelia strains we analyzed could be divided into two parts: the B. burgdorferi s.l. complex and the relapsing fever borreliae complex. TXW-1 segregated with the North American relapsing fever borreliae and formed a separate subbranch.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Arachnid Vectors / microbiology
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Borrelia / classification*
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Borrelia / genetics
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Borrelia / growth & development
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Borrelia / isolation & purification
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Borrelia burgdorferi / classification
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Borrelia burgdorferi / genetics
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Borrelia burgdorferi / isolation & purification
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Borrelia burgdorferi Group / classification*
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Borrelia burgdorferi Group / genetics
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Borrelia burgdorferi Group / growth & development
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Borrelia burgdorferi Group / isolation & purification
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DNA Fingerprinting
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DNA, Bacterial / analysis
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DNA, Bacterial / isolation & purification
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DNA, Ribosomal / analysis
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DNA, Ribosomal / isolation & purification
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Disease Reservoirs
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Genes, rRNA
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Genotype
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Lyme Disease / microbiology
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Molecular Sequence Data
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Phylogeny
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RNA, Ribosomal, 16S / genetics
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Random Amplified Polymorphic DNA Technique*
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Relapsing Fever / microbiology
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Sequence Analysis, DNA
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Southeastern United States
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Ticks / microbiology
Substances
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Ribosomal, 16S