C-reactive protein decreases prostacyclin release from human aortic endothelial cells

Circulation. 2003 Oct 7;108(14):1676-8. doi: 10.1161/01.CIR.0000094736.10595.A1. Epub 2003 Sep 22.

Abstract

Background: In addition to being a risk marker for cardiovascular disease, much recent data suggest that C-reactive protein (CRP) promotes atherogenesis. Decreased endothelial NO and prostacyclin (PGI2) contribute to a proatherogenic and prothrombotic state. We have shown that CRP decreases endothelial NO synthase expression and bioactivity in human aortic endothelial cells (HAECs). PGI2 is a potent vasodilator and inhibitor of platelet aggregation. Hence, the aim of this study was to examine the effect of CRP on PGI2 release from HAECs and human coronary artery endothelial cells (HCAECs).

Methods and results: HAECs and HCAECs were incubated with human CRP (0 to 50 microg/mL for 24 hours). The release of PGF-1alpha, a stable product of PGI2, was also assayed in the absence and presence of a potent agonist, A23187. CRP significantly decreased PGF-1alpha release from HAECs under basal (48% decrease, P<0.001; n=5) and stimulated (26% decrease, P<0.01; n=5) conditions. CRP had no effect on PGI2 synthase (PGIS) mass. By increasing both superoxide and inducible NO synthase, CRP resulted in increased nitration of PGIS by peroxynitrite. The increased nitration and decreased activity of PGIS by CRP was reversed with peroxynitrite scavengers.

Conclusions: Thus, CRP decreases PGI2 release from HAECs by inactivating PGIS via nitration, additionally contributing to its atherogenicity.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aorta / cytology
  • Aorta / metabolism*
  • Ascorbic Acid / pharmacology
  • C-Reactive Protein / pharmacology*
  • Cells, Cultured
  • Coronary Vessels / cytology
  • Coronary Vessels / metabolism
  • Cytochrome P-450 Enzyme System / chemistry
  • Cytochrome P-450 Enzyme System / metabolism
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism*
  • Epoprostenol / biosynthesis*
  • Free Radical Scavengers / pharmacology
  • Humans
  • Intramolecular Oxidoreductases / chemistry
  • Intramolecular Oxidoreductases / metabolism
  • Nitric Oxide Synthase / metabolism
  • Nitric Oxide Synthase Type II
  • Peroxynitrous Acid / metabolism
  • Prostaglandins F / biosynthesis
  • Tyrosine / analogs & derivatives*
  • Tyrosine / analysis
  • Uric Acid / pharmacology

Substances

  • Free Radical Scavengers
  • Prostaglandins F
  • Peroxynitrous Acid
  • Uric Acid
  • 3-nitrotyrosine
  • Tyrosine
  • C-Reactive Protein
  • Cytochrome P-450 Enzyme System
  • Epoprostenol
  • NOS2 protein, human
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type II
  • Intramolecular Oxidoreductases
  • prostacyclin synthetase
  • Ascorbic Acid
  • prostaglandin F1