Function of GATA transcription factors in preadipocyte-adipocyte transition

Science. 2000 Oct 6;290(5489):134-8. doi: 10.1126/science.290.5489.134.

Abstract

Genes that control the early stages of adipogenesis remain largely unknown. Here, we show that murine GATA-2 and GATA-3 are specifically expressed in white adipocyte precursors and that their down-regulation sets the stage for terminal differentiation. Constitutive GATA-2 and GATA-3 expression suppressed adipocyte differentiation and trapped cells at the preadipocyte stage. This effect is mediated, at least in part, through the direct suppression of peroxisome proliferator-activated receptor gamma. GATA-3-deficient embryonic stem cells exhibit an enhanced capacity to differentiate into adipocytes, and defective GATA-2 and GATA-3 expression is associated with obesity. Thus, GATA-2 and GATA-3 regulate adipocyte differentiation through molecular control of the preadipocyte-adipocyte transition.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Adipocytes / cytology*
  • Adipocytes / metabolism*
  • Adipose Tissue / cytology
  • Adipose Tissue / metabolism
  • Adipose Tissue, Brown / cytology
  • Adipose Tissue, Brown / metabolism
  • Animals
  • Cell Differentiation
  • Cells, Cultured
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • GATA2 Transcription Factor
  • GATA3 Transcription Factor
  • Gene Expression
  • Mice
  • Mutation
  • Obesity / genetics
  • Obesity / metabolism
  • Promoter Regions, Genetic
  • Receptors, Cytoplasmic and Nuclear / genetics
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Stem Cells / cytology
  • Trans-Activators / chemistry
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic
  • Zinc Fingers

Substances

  • DNA-Binding Proteins
  • GATA2 Transcription Factor
  • GATA3 Transcription Factor
  • Gata2 protein, mouse
  • Gata3 protein, mouse
  • Receptors, Cytoplasmic and Nuclear
  • Trans-Activators
  • Transcription Factors