Riboregulated toehold-gated gRNA for programmable CRISPR-Cas9 function

Ka-Hei Siu; Wilfred Chen

doi:10.1038/s41589-018-0186-1

Riboregulated toehold-gated gRNA for programmable CRISPR-Cas9 function

Nat Chem Biol. 2019 Mar;15(3):217-220. doi: 10.1038/s41589-018-0186-1. Epub 2018 Dec 10.

Authors

Ka-Hei Siu¹, Wilfred Chen²

Affiliations

¹ Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA.
² Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA. wilfred@udel.edu.

PMID: 30531984
DOI: 10.1038/s41589-018-0186-1

Abstract

Predictable control over gene expression is essential to elicit desired synthetic cellular phenotypes. Although CRISPR-Cas9 offers a simple RNA-guided method for targeted transcriptional control, it lacks the ability to integrate endogenous cellular information for efficient signal processing. Here, we present a new class of riboregulators termed toehold-gated gRNA (thgRNA) by integrating toehold riboswitches into sgRNA scaffolds, and demonstrate their programmability for multiplexed regulation in Escherichia coli with minimal cross-talks.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

CRISPR-Cas Systems / genetics
Clustered Regularly Interspaced Short Palindromic Repeats
Escherichia coli
Gene Editing / methods*
Gene Expression Regulation / physiology*
RNA, Guide, CRISPR-Cas Systems / genetics
RNA, Guide, CRISPR-Cas Systems / physiology
Riboswitch / genetics*
Riboswitch / physiology

Substances

RNA, Guide, CRISPR-Cas Systems
Riboswitch