Abstract
Oral antibodies that interfere with gastrointestinal targets and can be manufactured at scale are needed. Here we show that a single-gene-encoded monomeric immunoglobulin A (IgA)-like antibody, composed of camelid variable single domain antibodies (VHH) fused to IgA Fc (mVHH-IgA), prevents infection by enterotoxigenic Escherichia coli (F4-ETEC) in piglets. The mVHH-IgA can be produced in soybean seeds or secreted from the yeast Pichia pastoris, freeze- or spray-dried and orally delivered within food.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Administration, Oral
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Animals
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Communicable Diseases / drug therapy*
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Communicable Diseases / immunology
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Communicable Diseases / microbiology
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Escherichia coli / pathogenicity
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Food
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Gastrointestinal Diseases / drug therapy*
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Gastrointestinal Diseases / immunology
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Gastrointestinal Diseases / prevention & control
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Gastrointestinal Diseases / veterinary
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Humans
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Immunoglobulin A / immunology
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Immunoglobulin A / therapeutic use*
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Single-Domain Antibodies / immunology
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Single-Domain Antibodies / therapeutic use*
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Swine
Substances
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Immunoglobulin A
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Single-Domain Antibodies