Peptides with Potential Cardioprotective Effects Derived from Dry-Cured Ham Byproducts

J Agric Food Chem. 2019 Jan 30;67(4):1115-1126. doi: 10.1021/acs.jafc.8b05888. Epub 2019 Jan 16.

Abstract

The interest in using food byproducts as a source of bioactive peptides has increased significantly in the recent years. The goal of this work was to determine the presence and stability of peptides showing angiotensin I-converting enzyme (ACE-I), endothelin-converting enzyme (ECE), dipeptidyl peptidase-IV (DPP-IV), and platelet-activating factor-acetylhydrolase (PAF-AH) inhibitory activity derived from dry-cured ham bones, which could exert cardiovascular health benefits. ACE-I and DPP-IV inhibitory peptides were stable against heating typically used in Mediterranean household cooking methods and also to in vitro digestion. PAF-AH inhibitory activity significantly increased following simulated gastrointestinal digestion whereas ECE inhibitory significantly decreased ( P < 0.05). The mass spectrometry analysis revealed a notable degradation of hemoglobin-derived peptides after simulated digestion, and the release of a large number of dipeptides that may have contributed to the observed bioactivities. These results suggest that natural peptides from Spanish dry-cured ham bones could contribute to a positive impact on cardiovascular health.

Keywords: bioactive peptides; cooking; ham bones; in vitro digestion; mass spectrometry.

MeSH terms

  • Angiotensin-Converting Enzyme Inhibitors / chemistry*
  • Angiotensin-Converting Enzyme Inhibitors / isolation & purification
  • Animals
  • Bone and Bones / chemistry
  • Cardiotonic Agents / chemistry*
  • Cardiotonic Agents / isolation & purification
  • Cooking
  • Dipeptidyl Peptidase 4 / chemistry
  • Dipeptidyl-Peptidase IV Inhibitors / chemistry*
  • Dipeptidyl-Peptidase IV Inhibitors / isolation & purification
  • Endothelin-Converting Enzymes / chemistry
  • Kinetics
  • Meat Products / analysis*
  • Molecular Sequence Data
  • Peptide Mapping
  • Peptides / chemistry*
  • Peptides / isolation & purification
  • Peptidyl-Dipeptidase A / chemistry
  • Swine
  • Tandem Mass Spectrometry
  • Waste Products / analysis*

Substances

  • Angiotensin-Converting Enzyme Inhibitors
  • Cardiotonic Agents
  • Dipeptidyl-Peptidase IV Inhibitors
  • Peptides
  • Waste Products
  • Dipeptidyl Peptidase 4
  • Peptidyl-Dipeptidase A
  • Endothelin-Converting Enzymes