Abstract
We describe here the cloning and sequencing of human and mouse cDNAs encoding a putative GTP binding protein. Sequence comparison shows that these cDNAs (named eRFS) are likely to represent the orthologues of the yeast Saccharomyces cerevisiae HBS1 gene and that the C-terminal domains of the encoded proteins share structural features with eukaryotic elongation factor eEF-1A and release factor 3 (eRF3) families. The phylogenetic analysis suggests that eRFS proteins and Hbs1p form a cluster of orthologous sequences branching with the eRF3 family. Nevertheless, in yeast, the human eRFS protein and Hbs1p do not complement eRF3/Sup35p thermosensitive mutation and do not interact with eRF1.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Codon, Terminator
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DNA, Complementary / analysis
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Fungal Proteins / classification
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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GTP-Binding Proteins*
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Gene Expression
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HSP70 Heat-Shock Proteins / classification
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HSP70 Heat-Shock Proteins / genetics*
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HSP70 Heat-Shock Proteins / metabolism
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Humans
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Mice
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Molecular Sequence Data
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Mutation
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Peptide Elongation Factors*
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Peptide Termination Factors / classification
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Peptide Termination Factors / genetics*
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Peptide Termination Factors / metabolism
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Phylogeny
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Protein Biosynthesis
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RNA, Messenger / metabolism
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Saccharomyces cerevisiae / classification
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Sequence Analysis
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Sequence Homology, Amino Acid
Substances
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Codon, Terminator
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DNA, Complementary
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Fungal Proteins
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HBS1 protein, S cerevisiae
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HSP70 Heat-Shock Proteins
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Peptide Elongation Factors
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Peptide Termination Factors
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RNA, Messenger
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Saccharomyces cerevisiae Proteins
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peptide-chain-release factor 3
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GTP-Binding Proteins
Associated data
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GENBANK/AF087672
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GENBANK/U87791