Superclustering of B cell receptor and Fc gamma RIIB1 activates Src homology 2-containing protein tyrosine phosphatase-1

J Immunol. 1998 Sep 15;161(6):2716-22.

Abstract

Fc gamma RIIB1 (CD32) is a receptor that binds the Fc domain of Ag-complexed IgG. Coaggregation of B cell receptor (BCR) and Fc gamma RIIB1 generates a dominant negative signal that inhibits B cell activation. In Ag-specific Id-positive B cells, the co-cross-linking of BCR and Fc gamma RIIB1 by anti-Id Ab resulted in the association of both Src homology 2-containing protein tyrosine phosphatase (SHP-1) and Src homology 2-containing inositol phosphatase (SHIP) with the Fc gamma RIIB1; however, only SHIP activity was detected. "Superclustering" of the BCR and Fc gamma RIIB1 complex induced by stimulation with anti-Id Ab plus polyvalent Ag synergistically activated SHP-1. The degree of co-cross-linking between BCR and Fc gamma RIIB1 may determine the activation status of SHP-1 and SHIP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Anti-Idiotypic / pharmacology
  • Enzyme Activation / immunology
  • Haptens / genetics
  • Intracellular Signaling Peptides and Proteins
  • Lymphoma, B-Cell / immunology
  • Lymphoma, B-Cell / metabolism
  • Mice
  • Phosphorylation
  • Protein Phosphatase 1
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11
  • Protein Tyrosine Phosphatase, Non-Receptor Type 6
  • Protein Tyrosine Phosphatases / antagonists & inhibitors
  • Protein Tyrosine Phosphatases / metabolism*
  • Receptor Aggregation / immunology*
  • Receptors, Antigen, B-Cell / genetics
  • Receptors, Antigen, B-Cell / metabolism*
  • Receptors, Antigen, B-Cell / physiology
  • Receptors, IgG / metabolism*
  • Receptors, IgG / physiology
  • SH2 Domain-Containing Protein Tyrosine Phosphatases
  • Serum Albumin, Bovine / genetics
  • Serum Albumin, Bovine / immunology
  • Signal Transduction / immunology
  • Transfection / immunology
  • Trinitrobenzenes / immunology
  • Tumor Cells, Cultured
  • src Homology Domains / immunology*

Substances

  • Antibodies, Anti-Idiotypic
  • Haptens
  • Intracellular Signaling Peptides and Proteins
  • Receptors, Antigen, B-Cell
  • Receptors, IgG
  • Trinitrobenzenes
  • trinitrophenyl-bovine serum albumin
  • Serum Albumin, Bovine
  • Protein Phosphatase 1
  • Protein Tyrosine Phosphatase, Non-Receptor Type 11
  • Protein Tyrosine Phosphatase, Non-Receptor Type 6
  • Protein Tyrosine Phosphatases
  • Ptpn11 protein, mouse
  • Ptpn6 protein, mouse
  • SH2 Domain-Containing Protein Tyrosine Phosphatases