Abstract
Mutants of Bordetella pertussis which are defective in secretion of pertussis toxin were isolated and characterized. The region of the B. pertussis chromosome identified by mutagenesis as playing a role in transport of pertussis toxin was sequenced. Analysis of this region revealed eight open reading frames, seven of which predict a protein exhibiting homology with one of the VirB proteins of Agrobacterium tumefaciens, which are involved in the transport of the T-DNA molecule across bacterial and plant membranes. Thus a set of accessory proteins are most likely involved in the secretion of pertussis toxin, and these proteins appear to be members of a family of proteins involved in the secretion of macromolecules from bacteria.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Agrobacterium tumefaciens / genetics
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Amino Acid Sequence
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Bacterial Proteins / genetics
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Base Sequence
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Bordetella pertussis / genetics*
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Chromosomes, Bacterial*
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DNA, Bacterial / metabolism
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Kinetics
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Macromolecular Substances
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Molecular Sequence Data
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Mutagenesis
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Open Reading Frames
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Operon*
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Pertussis Toxin*
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Phenotype
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Sequence Homology, Amino Acid
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Virulence Factors*
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Virulence Factors, Bordetella / analysis
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Virulence Factors, Bordetella / biosynthesis
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Virulence Factors, Bordetella / genetics*
Substances
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Bacterial Proteins
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DNA, Bacterial
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Macromolecular Substances
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T-DNA
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Virulence Factors
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Virulence Factors, Bordetella
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Pertussis Toxin