Abstract
Interleukin-6 (IL-6), leukemia inhibitory factor, oncostatin M, interleukin-11, and ciliary neurotrophic factor bind to receptor complexes that share the signal transducer gp130. Upon binding, the ligands rapidly activate DNA binding of acute-phase response factor (APRF), a protein antigenically related to the p91 subunit of the interferon-stimulated gene factor-3 alpha (ISGF-3 alpha). These cytokines caused tyrosine phosphorylation of APRF and ISGF-3 alpha p91. Protein kinases of the Jak family were also rapidly tyrosine phosphorylated, and both APRF and Jak1 associated with gp130. These data indicate that Jak family protein kinases may participate in IL-6 signaling and that APRF may be activated in a complex with gp130.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD*
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Base Sequence
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Cytokine Receptor gp130
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Cytokines / pharmacology
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DNA-Binding Proteins / metabolism*
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Humans
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Interferon-Stimulated Gene Factor 3
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Interferon-Stimulated Gene Factor 3, gamma Subunit
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Interferon-gamma / pharmacology
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Interleukin-6 / pharmacology*
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Janus Kinase 1
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Membrane Glycoproteins / metabolism*
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Molecular Sequence Data
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Phosphorylation
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Protein-Tyrosine Kinases / metabolism*
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STAT1 Transcription Factor
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STAT3 Transcription Factor
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Signal Transduction
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Trans-Activators*
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Transcription Factors / metabolism
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Tumor Cells, Cultured
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Tyrosine / metabolism
Substances
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Antigens, CD
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Cytokines
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DNA-Binding Proteins
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IL6ST protein, human
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IRF9 protein, human
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Interferon-Stimulated Gene Factor 3
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Interferon-Stimulated Gene Factor 3, gamma Subunit
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Interleukin-6
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Membrane Glycoproteins
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STAT1 Transcription Factor
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STAT1 protein, human
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STAT3 Transcription Factor
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STAT3 protein, human
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Trans-Activators
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Transcription Factors
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Cytokine Receptor gp130
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Tyrosine
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Interferon-gamma
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Protein-Tyrosine Kinases
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JAK1 protein, human
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Janus Kinase 1