Molecular cloning of a cDNA encoding a novel small GTP-binding protein

FEBS Lett. 1993 Aug 9;328(1-2):21-4. doi: 10.1016/0014-5793(93)80957-v.

Abstract

A cDNA encoding a small GTP-binding protein, S10, was cloned from Jurkat cells. The deduced amino acid sequence of S10 had the structural features characteristic to this family of proteins with highest homology to rab subfamily. Northern blot analysis revealed that this gene is expressed only in lymphoid cell lines and a histiocytic leukemia, U937. Hence, it should have a specialized function in cells derived from the hematopoietic stem cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cell Line
  • Cells, Cultured
  • Cloning, Molecular
  • DNA / genetics*
  • DNA Probes
  • GTP-Binding Proteins / genetics*
  • Gene Expression Regulation
  • Humans
  • Macrophages / metabolism
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction
  • RNA / metabolism
  • Sequence Homology, Amino Acid
  • T-Lymphocytes / metabolism
  • Tumor Cells, Cultured

Substances

  • DNA Probes
  • RNA
  • DNA
  • GTP-Binding Proteins

Associated data

  • GENBANK/D14889