Highly-expressed lncRNA FOXD2-AS1 in adipose mesenchymal stem cell derived exosomes affects HaCaT cells via regulating miR-185-5p/ROCK2 axis

Adipocyte. 2023 Dec;12(1):2173513. doi: 10.1080/21623945.2023.2173513.

Abstract

The healing of skin wounds is a highly coordinated multi-step process that occurs after trauma including surgical incisions, thermal burns, and chronic ulcers. In this study, the authors investigated lncRNA FOXD2-AS1 function in adipose mesenchymal exosomes from ADMSCs that were successfully extracted. Highly expressed lncRNA FOXD2-AS1 in ADMSCs-exosomes accelerated HaCaT cell migration and proliferation. LncRNA FOXD2-AS1 negatively targeted miR-185-5p, and miR-185-5p negatively targeted ROCK2. Highly expressed lncRNA FOXD2-AS1 in ADMSCs-exosomes promoted HaCaT cell migration and proliferation via down-regulating miR-185-5p and further up-regulating ROCK2. In conclusion, LncRNA FOXD2-AS1 overexpression in ADMSCs derived exosomes might accelerate HaCaT cell migration and proliferation via modulating the miR-185-5p/ROCK2 axis.

Keywords: Exosomes; FOXD2-AS1; ROCK2; miR-185-5p; wound healing.

MeSH terms

  • Exosomes*
  • HaCaT Cells
  • Humans
  • Mesenchymal Stem Cells*
  • MicroRNAs* / genetics
  • RNA, Long Noncoding* / genetics
  • rho-Associated Kinases*

Substances

  • MicroRNAs
  • RNA, Long Noncoding
  • long non-coding RNA FOXD2-AS1, human
  • MIRN185 microRNA, human
  • ROCK2 protein, human
  • rho-Associated Kinases

Grants and funding

The author(s) reported that there is no funding associated with the work featured in this article.