Cyclophilin A accelerates SiO2-induced macrophage foaming

Heliang Liu; Hailan He; Ying Tian; Jie Cui; Shuang Wang; Hongli Wang

doi:10.1016/j.cellsig.2022.110562

Cyclophilin A accelerates SiO₂-induced macrophage foaming

Cell Signal. 2023 Mar:103:110562. doi: 10.1016/j.cellsig.2022.110562. Epub 2022 Dec 16.

Authors

Heliang Liu¹, Hailan He², Ying Tian², Jie Cui², Shuang Wang², Hongli Wang³

Affiliations

¹ School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063210, China; Hebei Key Laboratory of Organ Fibrosis, North China University of Science and Technology, Tangshan, Hebei 063210, China.
² School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063210, China.
³ School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063210, China. Electronic address: whllhl@163.com.

PMID: 36535629
DOI: 10.1016/j.cellsig.2022.110562

Abstract

Silicosis is a common occupational disease characterized by lung inflammation, fibrosis and pulmonary dysfunction caused by long-term inhalation of free SiO₂. Cell foaming and the change of CyPA have been observed in SiO₂-induced macrophages, but the specific mechanism of CyPA in SiO₂-induced foam cells remains poorly understood. The purpose of this study is to explore the mechanism of CyPA in SiO₂-induced macrophage foaming and its effect on silicosis. We found that overexpression of CyPA promoted the macrophage foaming and the expression of COL I and α-SMA, while silencing CyPA inhibites the macrophage foaming and the expression of COL I and α-SMA. After blocking the expression of CD36 on the basis of overexpression CyPA, we found it inhibites the macrophage foaming. In conclusion, CyPA can affect the foaming of macrophages and may participate in silicosis fibrosis.

Keywords: CD36; CyPA; Foam cells; Silicosis.

MeSH terms

Cyclophilin A* / metabolism
Foam Cells* / drug effects
Foam Cells* / enzymology
Humans
Pulmonary Fibrosis* / immunology
Silicon Dioxide* / toxicity
Silicosis* / immunology
Silicosis* / pathology

Substances

Cyclophilin A
Silicon Dioxide