Long non-coding RNA FEZF1-AS1 promotes rectal cancer progression by competitively binding miR-632 with FAM83A

Acta Biochim Biophys Sin (Shanghai). 2022 Apr 25;54(4):452-462. doi: 10.3724/abbs.2022022.

Abstract

The long non-coding RNA (lncRNA) forebrain embryonic zinc finger protein 1 antisense RNA1 (FEZF1-AS1) was recently identified as an oncogenic gene in several types of tumors. The biological function of FEZF1-AS1 in rectal cancer progression, however, remains unknown. In the present study, we discover that FEZF1-AS1 is significantly upregulated in rectal cancer tissues and cells. Knocking down of FEZF1-AS1 suppresses cell proliferation, migration, and invasion , and tumorigenesis . Furthermore, FEZF1-AS1 functions as a competing endogenous RNA (ceRNA) for miR-632, resulting in the suppression of family with sequence similarity 83, member A (FAM83A). Overall, our findings reveal that FEZF1-AS1/miR-632/FAM83A axis plays an oncogenic role in rectal cancer progression, suggesting that it may be a novel therapeutic target for rectal cancer.

Keywords: FAM83A; FEZF1-AS1; invasion; miR-632; migration; proliferation; rectal cancer.

MeSH terms

  • Carcinogenesis / genetics
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MicroRNAs* / genetics
  • Neoplasm Proteins* / genetics
  • RNA, Long Noncoding* / genetics
  • Rectal Neoplasms* / genetics

Substances

  • FAM83A protein, human
  • MIRN632 microRNA, human
  • MicroRNAs
  • Neoplasm Proteins
  • RNA, Long Noncoding

Grants and funding

This work was supported by the grants from the General Guidance Project of Hunan Provincial Health Commission (No. 20201937), the Clinical Medical Technology Innovation Guidance Project of Hunan Provincial Science and Technology Department (Nos. 2020SK51906 and 2018SK51701), the Provincial Natural Science Foundation of Hunan Provincial Science and Technology Department (Nos. 2021JJ30627 and 2020JJ4552), and the Major Research Projects of Hunan Provincial Health and Family Planning Commission (No. A2017012).