CircRAB3IP upregulates twist family BHLH transcription factor (TWIST1) to promote osteosarcoma progression by sponging miR-580-3p

Guojun Tang; Linghua Liu; Zhihong Xiao; Shuo Wen; Liangyuan Chen; Peng Yang

doi:10.1080/21655979.2021.1948487

CircRAB3IP upregulates twist family BHLH transcription factor (TWIST1) to promote osteosarcoma progression by sponging miR-580-3p

Bioengineered. 2021 Dec;12(1):3385-3397. doi: 10.1080/21655979.2021.1948487.

Authors

Guojun Tang¹, Linghua Liu², Zhihong Xiao¹, Shuo Wen³, Liangyuan Chen¹, Peng Yang⁴

Affiliations

¹ Department of Spine Surgery, The Second Affiliated Hospital, University of South China, Hengyang, Hunan, P.R. China.
² Department of Nursing, Hubei College of Chinese Medicine, Jingzhou, Hubei, P.R. China.
³ Department of Breast and Thyroid Surgery, Union Hospital West Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, P.R. China.
⁴ Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, P.R. China.

Abstract

Circular RNAs (circ RNAs) have been found to play an important role in cancer development. However, the role of circRAB3IP in osteosarcoma (OS) is unclear.In the present study, We found that circRAB3IP was highly expressed in OS tissues and OS cells. High levels of circRAB3IP was correlated with advanced TNM stage, distant metastasis. CircRAB3IP knockdown inhibited cell proliferation, migration, and invasion. Moreover, circRAB3IP directly binds to miR-580-3p. TWIST1 is directly targeted by miR-580-3p. We also demonstrated that circRAB3IP act as the sponge of miR-580-3p to promote TWIST1 expression. CircRAB3IP promotes OS cells proliferation, migration, and invasion through modulating miR-580-3p/TWIST1 axis. Moreover, circRAB3IP facilitated tumor formation in vivo. Our findings suggested that circRAB3IP acts as an oncogene in OS by regulating miR-580-3p/TWIST1 axis.

Keywords: Osteosarcoma; TWIST1; circRAB3IP; miR-580-3p.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Animals
Bone Neoplasms* / genetics
Bone Neoplasms* / metabolism
Bone Neoplasms* / pathology
Cell Line, Tumor
Child
Disease Progression
Female
Humans
Male
Mice
Mice, Inbred BALB C
MicroRNAs / genetics*
MicroRNAs / metabolism
Nuclear Proteins / genetics*
Nuclear Proteins / metabolism
Osteosarcoma* / genetics
Osteosarcoma* / metabolism
Osteosarcoma* / pathology
RNA, Circular / genetics*
RNA, Circular / metabolism
Twist-Related Protein 1 / genetics*
Twist-Related Protein 1 / metabolism
Young Adult

Substances

MIRN580 microRNA, human
MicroRNAs
Nuclear Proteins
RNA, Circular
TWIST1 protein, human
Twist-Related Protein 1

Grants and funding

This work was supported by the The Project supported by Wuhan Health Commission [WX18C29]; the National Natural Sciences Foundation of China [81401834].