Transcription factor activation and protein phosphorylation patterns are distinct for CD28 and ICAM-1 co-stimulatory molecules

Immunobiology. 2021 Mar;226(2):152067. doi: 10.1016/j.imbio.2021.152067. Epub 2021 Jan 28.

Abstract

We examined signaling differences between two co-stimulatory molecules, CD28 and ICAM-1 by analyzing transcription factors and proteins that are activated downstream of these co-stimulations. We observed that FAST-1, a crucial protein in the TGFβ signaling pathway, was activated by only ICAM-1 co-stimulation, and not by CD28. We also observed that receptor tyrosine kinases Csk, Dtk, FGFR1 and ROR2 were phosphorylated upon CD28 co-stimulation and IGF-1R, HGFR, MuSK and EphA8 were phosphorylated upon ICAM-1 co-stimulation. Together, these findings suggest that these two co-stimulators induce the activation of different sets of proteins, suggesting that each co-stimulatory molecule has its unique signaling profile.

Keywords: CD28; ICAM-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD28 Antigens / immunology*
  • CD4-Positive T-Lymphocytes / immunology
  • Humans
  • Intercellular Adhesion Molecule-1 / immunology*
  • Phosphorylation
  • Receptor Protein-Tyrosine Kinases / immunology*
  • Transcription Factors / immunology*

Substances

  • CD28 Antigens
  • Transcription Factors
  • Intercellular Adhesion Molecule-1
  • Receptor Protein-Tyrosine Kinases